Abstract
Salmonella enterica serovar Infantis (S. Infantis) is a globally distributed non-typhoid serovar infecting humans and food-producing animals. Considering the zoonotic potential and public health importance of this serovar, strategies to characterizing, monitor and control this pathogen are of great importance. This study aimed to determine the genetic relatedness of 80 Brazilian S. Infantis genomes in comparison to 40 non-Brazilian genomes from 14 countries using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR–Multi-Locus Virulence Sequence Typing (CRISPR–MVLST). CRISPR spacers were searched using CRISPR-Cas++ and fimH and sseL alleles using BLAST and MEGA X. Results were analyzed using BioNumerics 7.6 in order to obtain similarity dendrograms. A total of 23 CRISPR1 and 11 CRISPR2 alleles formed by 37 and 26 types of spacers, respectively, were detected. MVLST revealed the presence of five fimH and three sseL alleles. CRISPR’s similarity dendrogram showed 32 strain subtypes, with an overall similarity ≥ 78.6. The CRISPR–MVLST similarity dendrogram showed 37 subtypes, with an overall similarity ≥ 79.2. In conclusion, S. Infantis strains isolated from diverse sources in Brazil and other countries presented a high genetic similarity according to CRISPR and CRISPR–MVLST, regardless of their source, year, and/or place of isolation. These results suggest that both methods might be useful for molecular typing S. Infantis strains using WGS data.
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We would like to thank Editage (www.editage.com.br) for English language editing.
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This study was supported by research grants from the FDA/Center for Food Safety and Applied Nutrition (CFSAN) under the supervision of MWA and from the São Paulo Research Foundation (FAPESP; Proc. 2016/24716-3 and 2022/07013-0) under the supervision of JPF. FPV was supported by a Master’s student (2019/06947-6) and a PhD student scholarship from FAPESP (2021/07365–0). JPF was supported by a Productive fellowship (Proc. 304399/2018-3 and 304803/2021-9) of the National Council for Scientific and Technological Development. This study was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil—Finance Code 001.
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Vilela, F.P., Rodrigues, D.d., Allard, M.W. et al. CRISPR and CRISPR–MVLST reveal conserved spacer distribution and high similarity among Salmonella enterica serovar Infantis genomes from Brazil and other countries. Mol Genet Genomics 299, 61 (2024). https://doi.org/10.1007/s00438-024-02147-0
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DOI: https://doi.org/10.1007/s00438-024-02147-0