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Microbial community composition associated with early developmental stages of the Indian white shrimp, Penaeus indicus

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Abstract

Gut microbiota is known to influence the physiology, health, nutrient absorption, reproduction, and other metabolic activities of aquatic organisms. Microbial composition can influence intestinal immunity and are considered as health indicators. Information on gut microbial composition provides potential application possibilities to improve shrimp health and production. In the absence of such information for Penaeus indicus, the present study reports the microbial community structure associated with its early developmental stages. Bacterial community associated with the early developmental stages (egg, nauplii, zoea, mysis, PL1, PL6 and PL12) from two hatchery cycles were analysed employing 16S rRNA high throughput sequencing. Proteobacteria and Bacteroidetes, were the two dominant phyla in P. indicus development stages. Sequential sampling revealed the constant change in the bacterial composition at genus level. Alteromonas was dominant in egg and nauplii stage, whilst Ascidiaceihabitans (formerly Roseobacter) was the dominant genera in both PL6 and PL12. The bacterial composition was highly dynamic in early stages and our study suggests that the mysis stage is the critical phase in transforming the microbial composition and it gets stabilised by early post larval stages. This is the first report on the composition of microbiota in early developmental stages of P. indicus. Based on these results the formation of microbial composition seems to be influenced by feeding at early stages. The study provides valuable information to device intervention strategies for healthy seed production.

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The original contributions presented in the study are included in the article/Supplementary Information; further inquiries can be directed to the corresponding author.

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Acknowledgements

Authors acknowledge the facilities and support provided by the Director, ICAR-CIBA to carry out this work through the institute funded project FISHCIBASIL201800200130 & Consortia Research Platform (CRP) on Vaccine and Diagnostics (CRP-V&D) 1007611.

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Author notes

  1. T. N. Vinay and P. K. Patil have contributed equally and share first authorship.

Authors and Affiliations

  1. ICAR-Central Institute of Brackishwater Aquaculture, 75, Santhome High Road, MRC Nagar, Chennai, 600028, India

    T. N. Vinay, P. K. Patil, R. Aravind, P. S. Shyne Anand, V. Baskaran & C. P. Balasubramanian

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  1. T. N. Vinay
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  2. P. K. Patil
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  3. R. Aravind
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  4. P. S. Shyne Anand
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  5. V. Baskaran
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  6. C. P. Balasubramanian
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Contributions

PKP and VTN: planning of the study, PKP: critical evaluation and fund mobility. VTN, AR, and SAPS: shrimp seed production and larval rearing, VTN and VB: sampling and Sequencing. VTN: Metagenomic analysis and data interpretation. PKP and VTN wrote the manuscript, CPB: reviewed and provided valuable inputs to the manuscript. All authors have read and approved the final manuscript.

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Correspondence to T. N. Vinay.

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Communicated by Martine Collart.

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Figure S1

Linear discriminative analysis (LDA) effect size (LEfSe) of potential taxonomic biomarkers among different developmental stages of P. indicus. 34 genera, varying significantly were identified with LDA scores >4.. Supplementary file1 (PDF 17 KB)

Supplementary file2 (XLSX 6861 KB)

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Vinay, T.N., Patil, P.K., Aravind, R. et al. Microbial community composition associated with early developmental stages of the Indian white shrimp, Penaeus indicus. Mol Genet Genomics 297, 495–505 (2022). https://doi.org/10.1007/s00438-022-01865-7

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  • DOI: https://doi.org/10.1007/s00438-022-01865-7

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