Abstract
Twelve different Ty1-copia and Ty3-gypsy group LTR retrotransposons were compared for their usefulness in SSAP marker development in two agriculturally important Vicia species. Three of the retrotransposons, PDR1, Tps19 and Tvf4, yielded useful SSAP marker systems in V. faba, and V. narbonensis. Another, Tvf1 was a good source of SSAP markers in V. narbonensis alone. The optimized SSAP marker systems were applied to the analysis of two diverse Vicia germplasm sets. Two hundred and two polymorphic Tvf1 SSAP markers were scored in 56 V. narbonensis samples and 196 polymorphic markers derived from the other three most useful retrotransposons were scored in a collection of 20 V. faba samples. The marker data were then used to construct phylogenetic trees. The trees for both species tend to show long-branch lengths, with rather little fine structure. Some V. narbonensis accessions cluster by geographical origin but many do not and a given geographical region is often represented by multiple diverse groups in the tree, suggesting a deep and ancient structure for the diversity of V. narbonensis that spans its current geographic range. The tree for the V. faba accessions also shows very limited clustering with geographical origin and no obvious correlation between diversity and morphology-based taxonomic groupings for the species.
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Acknowledgments
This work was supported by Grant FP6-2002-FOOD-1-506223 (Grain Legumes) from the European Commission under the Framework VI Programme and Spanish National Project INTERREG MEJLEG/SP2.E40/02. We are very grateful to Ana Maria Torres Romero for DNA samples, information and advice. We also thank Noel Ellis and Frederic Muel for advice, suggestions and encouragement. AMS was supported by a grant from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) and SGG was supported by Complementary aid from the Fellowship by Consejeria de Agricultura y Pesca of Junta de Andalucia.
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Authors Alberto Martín Sanz, Susana Gilsanz Gonzalez and Naeem H. Syed have made equal contributions.
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Sanz, A.M., Gonzalez, S.G., Syed, N.H. et al. Genetic diversity analysis in Vicia species using retrotransposon-based SSAP markers. Mol Genet Genomics 278, 433–441 (2007). https://doi.org/10.1007/s00438-007-0261-x
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DOI: https://doi.org/10.1007/s00438-007-0261-x