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Different infective forms trigger distinct lesions in the colon during experimental Chagas disease

  • Immunology and Host-Parasite Interactions - Original Paper
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Abstract

With the control of vectorial transmission of Chagas disease caused by metacyclic trypomastigotes (MT) in endemic countries, other pathways of infection have become important. The infection caused by blood trypomastigotes (BT) is relevant in places where the blood transfusion and organ transplantation are poorly controlled. This study aimed to evaluate immunopathogenic parameters in the colon during the acute and chronic phases of experimental infection in Swiss mice infected with BT or MT forms of VL-10 strain of Trypanosoma cruzi. We have found that animals infected with MT forms presented lower survival rate, and higher tissue parasitism in the acute phase of the disease, which may be associated with the exacerbated activation of the immune system with the production of pro-inflammatory cytokines even in the chronic phase of infection. Taken together, these results can also be associated to the maintenance of the inflammatory process in chronic phase and an earlier denervation of myenteric plexus in colon. These findings emphasized the importance of the inoculum source and the strain, once different forms of different strains seem to promote distinct diseases.

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Data availability

The datasets generated and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

We are thankful to the Animal Facility at Ouro Preto Federal University (UFOP), Minas Gerais State, Brazil (CCA/UFOP), for providing the animals and multi-user microscopy and flow cytometry facilities from Nucleus of Biological Sciences Research – NUPEB-UFOP for the contribution with morphometric and cytometric analysis.

Funding

This work was supported by the Universidade Federal de Ouro Preto (PROPPI-UFOP), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Authors and Affiliations

  1. Laboratory of Morphopathology, Department of Biological Sciences, Nucleus of Biological Sciences Research, Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil

    Flávia de Souza Marques, Thays Helena Chaves Duarte, Viviane Flores Xavier, Aline Tonhela Ferraz, Aline Coelho das Mercês, Thaís Vieira Carvalho Silva & Paula Melo de Abreu Vieira

  2. Laboratory of Immunopathology, Nucleus of Biological Sciences Research, Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil

    Lívia Carvalho Mendes, Luciana da Fonseca Medeiros, Luísa Perin, Fernando Augusto Siqueira Mathias, Kátia da Silva Fonseca, Nivia Carolina Nogueira-Paiva & Cláudia Martins Carneiro

  3. Department of Clinical Analysis, School of Pharmacy, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil

    Cláudia Martins Carneiro

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  1. Flávia de Souza Marques
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  2. Thays Helena Chaves Duarte
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Correspondence to Paula Melo de Abreu Vieira.

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de Souza Marques, F., Duarte, T.H.C., Xavier, V.F. et al. Different infective forms trigger distinct lesions in the colon during experimental Chagas disease. Parasitol Res 120, 3475–3486 (2021). https://doi.org/10.1007/s00436-021-07236-2

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