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Susceptibility, metacercarial burden, and mortality of juvenile silver barb, common carp, mrigal, and tilapia following exposure to Haplorchis taichui

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Abstract

Fish-borne zoonotic trematodes (FZT) including heterophyids and opisthorchiids are prevalent in native and aquaculture fish in Southeast Asia. FZT are reported highly prevalent in juvenile aquaculture fish that belonged to the family Heterophyidae, particularly Haplorchis taichui. Aquaculture fish are reported to have varying levels of natural infection with H. taichui, but data for their susceptibility as well as resistance to infection are not available. The aim of this study was to determine the susceptibility of five aquaculture fish to H. taichui. Experimental infections were performed by exposing fish individually to 0 (control), 50, 100, or 200 H. taichui cercariae for 12 h. Metacercarial burden was measured at 45 days postinfection. Three out of five fish species, silver barb (Barbonymus gonionotus), common carp (Cyprinus carpio), and mrigal (Cirrhinus mrigala), were highly susceptible to H. taichui infection with percentage ranging from 93.33 to 100 %. The Nile and red tilapia were not susceptible to infection. Among the susceptible fish species, silver barb had higher intensity of H. taichui metacercariae than common carp and mrigal (P < 0.001). Metacercarial burden significantly correlated with the dose of cercarial infection (P < 0.001). Our findings that common aquaculture fish species have varying degrees of susceptibility to H. taichui infection provide important information to reduce parasite transmission in aquaculture fish.

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Acknowledgments

We acknowledge the support of the Faculty of Medicine Khon Kaen University, Visiting International Professor Program and the Cholangiocarcinoma Screening and Care Program (CASCAP), Khon Kaen University, 40002 Thailand. This work was supported by the Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University and Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, through health cluster (SHeP-GMS).

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand

    Kulthida Kopolrat, Paiboon Sithithaworn, Smarn Tesana & Ross H. Andrews

  2. Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand

    Kulthida Kopolrat, Paiboon Sithithaworn & Ross H. Andrews

  3. Cholangiocarcinoma Screening and Care Program (CASCAP), Khon Kaen University, Khon Kaen, 40002, Thailand

    Ross H. Andrews & Trevor N. Petney

  4. Imperial College London, Faculty of Medicine, St Mary’s Campus, South Wharf Street, London, W2 1NY, UK

    Ross H. Andrews

  5. Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany

    Trevor N. Petney

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  1. Kulthida Kopolrat
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  2. Paiboon Sithithaworn
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Correspondence to Paiboon Sithithaworn.

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Kopolrat, K., Sithithaworn, P., Tesana, S. et al. Susceptibility, metacercarial burden, and mortality of juvenile silver barb, common carp, mrigal, and tilapia following exposure to Haplorchis taichui . Parasitol Res 114, 1433–1442 (2015). https://doi.org/10.1007/s00436-015-4326-1

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