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Molecular characterization of Trypanosoma cruzi sylvatic isolates from Rio de Janeiro, Brazil

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Abstract

Trypanosoma cruzi, the etiologic agent of Chagas disease, presents considerable heterogeneity between isolated populations within the wild and domestic cycles. By using multiplex polymerase chain reaction based on the mini-exon gene, characterization was performed on seven samples isolated from specimens of Triatoma vitticeps that had been collected from the locality of Triunfo in the municipality of Santa Maria Madalena, state of Rio de Janeiro, Brazil. The samples SMM10, SMM53, SMM88, and SMM98 (area A) and SMM36 and SMM82 (area B) revealed the presence of 150 base pairs, corresponding to the zymodeme III (Z3). Our study suggested that one isolate (SMM1) presents a mixed genotype associated with Z3 and TcII. The typing of isolates of T. cruzi has the main aim of identifying strains with different epidemiological and/or clinical characteristics of Chagas disease. Our results corroborate other descriptions in the literature and contribute towards the knowledge and records of the profile of some additional wild isolates of T. cruzi in regions not yet affected by the disease.

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Acknowledgments

We would like to thank the Laboratório de Doenças Parasitarias and Departamento de Medicina Tropical, Instituto Oswaldo Cruz (FIOCRUZ). This study is supported by grants from Fundação de Amparo a Pesquisa do Rio de Janeiro (FAPERJ) and FIOCRUZ, Rio de Janeiro, Brazil.

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Authors and Affiliations

  1. Setor de Morfologia, Ultra-estrutura e Bioquímica de Artrópodes e Parasitos/Laboratório de Transmissores de Leishmanioses, Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, CEP: 21040-900, Rio de Janeiro, Brazil

    Jacenir R. Santos-Mallet, Cristina S. Silva, Suzete A. O. Gomes, Daniele M. Sousa & Teresa Cristina M. Gonçalves

  2. Laboratório de Doenças Parasitárias, Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, CEP: 21040-900, Rio de Janeiro, RJ, Brazil

    Daise L. Oliveira, Cristina L. Santos & Angela C. V. Junqueira

  3. Programa de Pós-graduação em Biologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ, Brazil

    Nadja L. Pinheiro

Authors
  1. Jacenir R. Santos-Mallet
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  2. Cristina S. Silva
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  3. Suzete A. O. Gomes
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  4. Daise L. Oliveira
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  5. Cristina L. Santos
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  6. Daniele M. Sousa
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  7. Nadja L. Pinheiro
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  8. Angela C. V. Junqueira
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  9. Teresa Cristina M. Gonçalves
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Correspondence to Jacenir R. Santos-Mallet.

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Santos-Mallet, J.R., Silva, C.S., Gomes, S.A.O. et al. Molecular characterization of Trypanosoma cruzi sylvatic isolates from Rio de Janeiro, Brazil. Parasitol Res 103, 1041–1045 (2008). https://doi.org/10.1007/s00436-008-1089-y

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  • DOI: https://doi.org/10.1007/s00436-008-1089-y

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