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High intragenotypic diversity of Giardia duodenalis in dairy cattle on three farms

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Abstract

Fifty-eight Giardia duodenalis-positive fecal specimens from three dairy farms in Georgia, USA were genotyped and subtyped by sequence analysis of the triosephosphate isomerase gene. Both the livestock-specific assemblage E and the potentially zoonotic assemblage A were found, with the former assemblage detected in 83% of the specimens. A high degree of genetic polymorphism was evident within assemblage E, with 11 distinct subtypes identified, eight of which represented new subtypes. Three subtypes were identified in assemblage A, with the subtype A2 transiently found in calves and cows on one farm. All farms had multiple assemblage E subtypes circulating in cattle at each sampling, and concurrent infection with mixed subtypes or genotypes occurred in 24% of animals. Thus, the high intensity of G. duodenalis transmission is not only reflected by the high prevalence of the infection but also by the high intragenotypic diversity and concurrent occurrence of mixed infections. The zoonotic potential of bovine G. duodenalis needs to be further studied by extensive characterization of assemblage A specimens at the subtype level.

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Acknowledgments

This study was supported, in part, by the College of Agriculture and Environmental Sciences Seed Grant, University of Georgia and the National Natural Science Foundation of China (No. 30771881). Yaoyu Feng was further supported by a grant from the Program for New Century Excellent Talents in Chinese Universities (NCET-05-0382) and an Emerging Infectious Diseases Fellowship from the Centers for Disease Control and Prevention and the Association of Public Health Laboratories. We thank Alison Payton and the CFS Parasitology Laboratory staff for sampling and technical support.

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Correspondence to Lihua Xiao.

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The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

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Feng, Y., Ortega, Y., Cama, V. et al. High intragenotypic diversity of Giardia duodenalis in dairy cattle on three farms. Parasitol Res 103, 87–92 (2008). https://doi.org/10.1007/s00436-008-0932-5

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  • DOI: https://doi.org/10.1007/s00436-008-0932-5

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