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14-3-3 gene characterization and description of a second 14-3-3 isoform in both Echinococcus granulosus and E. multilocularis

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Abstract

Members of the 14-3-3 protein family have been identified as regulatory molecules in intracellular signaling pathways and cell cycle control. Previously, the first Echinococcus 14-3-3 isoform (E14-3-3.1) was isolated from E. granulosus and E. multilocularis metacestode stages. Hyperexpression of this isoform was claimed to be associated with non-restricted tumor-like growth of the E. multilocularis metacestode. In this report, we describe the characterization of a 14-3-3 cDNA from E. granulosus and E. multilocularis corresponding to a second isoform of this family, E14-3-3.2. The characterized 14-3-3 gene was interrupted by two introns whose sequence and positions were conserved in both Echinococcus species. The deduced amino acid sequence of E14-3-3.2 showed 88% identity to the E14-3-3.1 isoform and 52% identity to a third Echinococcus isoform (E14-3-3.3) described by other authors. These findings, coupled to Southern blot analysis, suggest the presence of more than one 14-3-3 gene in Echinococcus. Phylogenenetically, the Echinococcus 14-3-3.1 and 14-3-3.2 isoforms appeared to cluster with zeta-type (“pro-tumorigenic”) 14-3-3 isoforms from closely related organisms, whereas the E14-3-3.3 isoform grouped with 14-3-3 epsilon isoforms. The presence of more than one 14-3-3 isoform might indicate isoform-specific roles in the different parasite stages of Echinococcus.

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Acknowledgements

This work was supported by PADCT (620081/95-3), CNPq, FAPERGS, the Swiss National Science Foundation (Project 31-63615.00) and the Ministry of Science and Technology in Spain (Project AGL2002-00644). M.S.L. is the holder of a “Ramón y Cajal” contract. We acknowledge the financial contributions made by the Interreg II project BWA 30.027 and the EU EchinoRisk project QLK2-CT-2001-01995 (BBW 00.0586-1) and also thank J.J. Arenzon for a critical reading of the manuscript and A.P. Nunes for help with the figures.

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Authors and Affiliations

  1. Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, RS 91501-970, Porto Alegre, Brazil

    Cláudia Paiva Nunes & Arnaldo Zaha

  2. Institute of Parasitology, University of Berne, Switzerland

    Bruno Gottstein & Norbert Müller

  3. Unidad de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Spain

    María del Mar Siles-Lucas

Authors
  1. Cláudia Paiva Nunes
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  2. Arnaldo Zaha
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  3. Bruno Gottstein
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  4. Norbert Müller
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  5. María del Mar Siles-Lucas
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Correspondence to Cláudia Paiva Nunes.

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Nunes, C.P., Zaha, A., Gottstein, B. et al. 14-3-3 gene characterization and description of a second 14-3-3 isoform in both Echinococcus granulosus and E. multilocularis. Parasitol Res 93, 403–409 (2004). https://doi.org/10.1007/s00436-004-1147-z

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  • DOI: https://doi.org/10.1007/s00436-004-1147-z

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