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Increased expressions of NKp44, NKp46 on NK/NKT-like cells are associated with impaired cytolytic function in self-limiting hepatitis E infection

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Abstract

We have characterized the NK/NKT-like cells in patients with self-limiting hepatitis E infection. The distribution of peripheral NK/NKT-like cells, expressions of activation receptors, cytotoxic potential and effector function of NK/NKT-like cells from fresh peripheral blood mononuclear cells of 86 acute patients, 101 recovered and 54 control individuals were assessed. Activated NKT-like (CD16+ CD56+ CD3+) cells were high in the patient groups. On CD56+ CD3− cells, NKp44 and NKp46 expressions were high in the acute patients, whereas NKp30, NKp44, NKp46 and NKG2D were high in the recovered individuals. On CD56+ CD3+ cells, NKp44, NKp46 and NKG2D expressions were high in the recovered but NKp30 was low in both the patient groups. Collectively, the current study elucidates the role of NK/NKT-like cells demonstrating phenotypic alterations of activated NKT-like cells and activation receptors, lack of CD107a expression and functional impairment of peripheral NK/NKT-like cells in self-limiting hepatitis E infection.

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Acknowledgments

We thank Director, National Institute of Virology, for all the encouragements. Special thanks are due to Mr. Prasad Babbar, Mr. Vasant Walkoli, Mr. Bipin Tilekar, Mr. Sarang Kamble, Mr. Dasharath, Mr. Prakash Sarje, Mr. Prakash B. Jawalkar, Ms. Neeta Thorat and Mr. Shirish Vaidya for technical help. Contributions of Mr. Kanad Madansure, Mr. Chinmaya Mahapatra, Mr. Rishikesh Lotke and Ms. Aparna Hande are greatly acknowledged. We thank Mr. Sanjay Rathod and Mr. Subrat Thanapati for their timely help and critical reading the manuscript. We thank Dr. A. L. Kakrani (D. Y. Patil Medical College, Pune, India) and Dr. Madhuri Thakar (NARI, Pune, India) for their encouragements.

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  1. Hepatitis Group, MCC, National Institute of Virology, 130/1, Sus Road, Pashan, Pune, 411021, Maharashtra, India

    Rumki Das & Anuradha Tripathy

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  1. Rumki Das
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Correspondence to Anuradha Tripathy.

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Das, R., Tripathy, A. Increased expressions of NKp44, NKp46 on NK/NKT-like cells are associated with impaired cytolytic function in self-limiting hepatitis E infection. Med Microbiol Immunol 203, 303–314 (2014). https://doi.org/10.1007/s00430-014-0338-1

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  • DOI: https://doi.org/10.1007/s00430-014-0338-1

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