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Generation of genetic engineering monoclonal antibodies against prion protein

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Abstract

Two strains of Fab monoclonal antibodies (mAbs) against prion protein, designated as IV-66 and IV-78, were selected from the phage display libraries. The gene sequences encoding the light κ chain and heavy Fd chain of IV-78 were inserted into a baculovirus expression cassette vector for mouse IgG expression. Western blot, Dot-ELISA and immunoprecipitation confirmed that these Fab and IgG mAbs reacted well with the recombinant hamster and human PrP proteins expressed in prokaryotic and in mammalian cells and PrPSc from scrapie-infected hamsters. It demonstrates that mAbs against prion protein are successfully generated by phage-display technique.

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Acknowledgments

This work was supported by Chinese National Natural Science Foundation Grants 30571672 and 30500018, National Science and Technology Task Force Project (2003BA712A04-02) and EU Project QLRT 2000 01441.

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Authors and Affiliations

  1. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Rd 100, Beijing, 100052, People’s Republic of China

    Yin-Xia Huang, Jun Han, Chen-Fang Dong, Li Sun, Chen Gao, Xiao-Fan Wang, Lu Han, Wei Zhou, Bao-Yun Zhang, Hui-Ying Jiang, Mi-Fang Liang & Xiao-Ping Dong

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  1. Yin-Xia Huang
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  2. Jun Han
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  3. Chen-Fang Dong
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  4. Li Sun
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  6. Xiao-Fan Wang
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  7. Lu Han
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Correspondence to Xiao-Ping Dong.

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Huang, YX., Han, J., Dong, CF. et al. Generation of genetic engineering monoclonal antibodies against prion protein. Med Microbiol Immunol 196, 241–246 (2007). https://doi.org/10.1007/s00430-007-0049-y

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  • DOI: https://doi.org/10.1007/s00430-007-0049-y

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