Abstract
Coleoid cephalopods have a high intelligence, complex structures, and large brain. The cephalopod brain is divided into supraesophageal mass, subesophageal mass and optic lobe. Although much is known about the structural organization and connections of various lobes of octopus brain, there are few studies on the brain of cephalopod at the molecular level. In this study, we demonstrated the structure of an adult Octopus minor brain by histomorphological analyses. Through visualization of neuronal and proliferation markers, we found that adult neurogenesis occurred in the vL and posterior svL. We also obtained specific 1015 genes by transcriptome of O. minor brain and selected OLFM3, NPY, GnRH, and GDF8 genes. The expression of genes in the central brain showed the possibility of using NPY and GDF8 as molecular marker of compartmentation in the central brain. This study will provide useful information for establishing a molecular atlas of cephalopod brain.
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Data availability
The RNA-seq raw data used in this study have been deposited in the Zenodo database under accession code https://doi.org/10.5281/zenodo.7701881. Related sequencing data have been uploaded to NCBI’s GenBank (BTG1, OP751951; SerT, OP751952; OLFM3, OP751948; NPY, OP751944; GnRH, OP751945; GDF8, OP751949). Other data supporting the findings of the present study are available from the corresponding author upon reasonable request.
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This work was supported by the research grant of the Chungbuk National University in 2021.
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All authors contributed to the study conception and design. C-JL, H-YL, Y-CG, SS, and S-JC contributed to experiments, material preparation, data collection, and analysis. Y-SY DEG contributed to analysis. HL and KK contributed to western blotting. C-JL, K-BR contributed to experiments and prepared the figures. First draft of the manuscript was written by C-JL, H-YL, Y-CG, SS and S-JC, S-JC supervised the project. All authors read and approved the final manuscript.
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Based on ‘The regulation of IACUC in CBNU (Chungbuk national University)’ and ‘The related laws (animal welfare act, laboratory animal act)’, the IACUC in CBNU require an animal use protocol for experiments involving vertebrate animals. However, it does not require animal use protocol for experiments using invertebrate species (e.g., brine shrimp, fruit flies, nematodes, Cephalopoda, etc.). Therefore, the review of the animal use protocol by IACUC is not mandatory for this research. We have uploaded a specific waiver letter-stamped by the ethics committee (CBNU) as Supplementary Material.
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Lee, CJ., Lee, HY., Yu, YS. et al. Brain compartmentalization based on transcriptome analyses and its gene expression in Octopus minor. Brain Struct Funct 228, 1283–1294 (2023). https://doi.org/10.1007/s00429-023-02647-6
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DOI: https://doi.org/10.1007/s00429-023-02647-6