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Association of a p73 exon 2 GC/AT polymorphism with colorectal cancer risk and survival in Tunisian patients

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Abstract

We examined the association of one linked GC/AT polymorphism at p73 with the risk of colorectal cancer. In the present study, we investigated whether this polymorphism was related to the risk of colorectal cancer, and whether there were relationships between the polymorphism and LOH, protein expression or clinicopathological variables. The p73 genotypes were determined by PCR-restriction fragment length polymorphism in 150 Tunisians patients with colorectal cancer and in 204 healthy control subjects. Immunohistochemistry was performed on normal mucosa, primary tumour and metastasis. The frequencies of the genotypes were 52% for wild-type (GC/GC), 31% for heterozygotes (GC/AT) and 17% for variants (AT/AT) in patients, and 54%, 35% and 11% in controls, respectively. There were no significant differences of the frequencies of the three genotypes between the patients and controls (p = 0.11). We did not find any relationship of the genotypes with clinicopathological features of patients. We found that patients with the AT/AT genotype had a significantly worse clinical outcome than those with the GC/AT and GC/GC genotype. There were no significant differences between tumoural immunostaining of the total p73 and p73 polymorphism (p = 0.16). However, we found a significant difference between the expression profile of ΔNp73 isoform and frequencies of the three genotypes (p = 0.0001). No LOH was observed at p73 locus. Our results suggest that the AT/AT genotype is significantly associated with poor prognosis in colorectal cancer. All these findings suggest that p73 polymorphism analysis may provide useful prognostic information for colorectal cancer patients.

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Acknowledgment

This study was supported by a grant from the Ministry of the technology and the scientific research.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Colorectal Cancer Research UR03ES04, Science University Tunis, Tunis, Tunisia

    Amira Toumi Arfaoui, Lilia Ben Mahmoud Kriaa, Myriam Khiari & Sadaa Bouraoui

  2. Department of Pathology and Cytology, Mongi Slim Hospital La Marsa, Tunis, Tunisia

    Olfa El Amine El Hadj, Sabeh Mzabi & Sadaa Bouraoui

  3. Department of Epidemiology and Preventive Medicine, Medecine University Tunis, Tunis, Tunisia

    Majid A. Ben Hmida

  4. Department of General Surgery, Mongi Slim Hospital La Marsa, Tunis, Tunisia

    T. Khalfallah & Lasaad Gharbi

  5. Department of Pathology and Cytology, Mongi Slim Hospital, Sidi Daoud, 2047, Tunis, Tunisia

    Amira Toumi Arfaoui

  6. 4-1 Res Semiramis Apt Bader El Boudour Borj El Wezir, 2036, La Soukra, Tunisia

    Amira Toumi Arfaoui

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  1. Amira Toumi Arfaoui
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  2. Lilia Ben Mahmoud Kriaa
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  9. Sadaa Bouraoui
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Correspondence to Amira Toumi Arfaoui.

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Arfaoui, A.T., Kriaa, L.B.M., El Hadj, O.E.A. et al. Association of a p73 exon 2 GC/AT polymorphism with colorectal cancer risk and survival in Tunisian patients. Virchows Arch 457, 359–368 (2010). https://doi.org/10.1007/s00428-010-0942-4

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  • DOI: https://doi.org/10.1007/s00428-010-0942-4

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