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Analysis of chromosome-11 aberrations in pulmonary and gastrointestinal carcinoids: an array comparative genomic hybridization-based study

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Abstract

Carcinoid tumors are a heterogeneous group of neoplasms arising from the diffuse neuroendocrine system. Pulmonary as well as gastrointestinal carcinoids can be separated into those with low malignant and intermediate malignant potential. DNA losses of chromosome arm 11q are commonly seen in pulmonary neuroendocrine tumors. Conflicting results have been published comparing atypical with typical lung carcinoids with respect to imbalances of chromosome 11. In the present study, a DNA microarray with genomic clones mapped to chromosome 11 was created, and array comparative genomic hybridization (CGH) with DNA derived from formalin-fixed, paraffin-embedded tissue was performed. We investigated 4 typical and 12 atypical carcinoids of the lung and, for comparison, 9 gastrointestinal carcinoids and 6 endocrine pancreatic tumors. We have shown that formalin-fixed, paraffin-derived DNA can be successfully used for array CGH. Alterations of 11q were rarely detected not only in typical carcinoids of the lung but also in gastrointestinal carcinoids. Atypical lung carcinoids that comprised extensive DNA losses also presented retained fragments in between these deleted regions. The array CGH data were consistent with the data of a previously published classical CGH study and were additionally confirmed using fluorescence in situ hybridization in the present investigation.

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Acknowledgments

We thank Dr. Mariano Rocchi, Resources for Molecular Cytogenetics, for providing us with BAC/PAC clones of chromosome 11. We would like to thank the Mapping Core and Map Finishing groups of the Wellcome Trust Sanger Institute for initial clone supply and verification. We thank Margit Gogg-Kammerer for her technical assistance. This work was supported by the Austrian National Bank Jubilee Fund, Project 8454 and the Austrian Research Fund, Project P14759.

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Authors and Affiliations

  1. Institute of Pathology, Medical School Graz, Austria

    Susanna Petzmann, Reinhard Ullmann, Iris Halbwedl & Helmut H. Popper

  2. Institute of Pathology, Auenbruggerplatz 25, 8036 , Graz, Austria

    Helmut H. Popper

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  1. Susanna Petzmann
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Correspondence to Helmut H. Popper.

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Petzmann, S., Ullmann, R., Halbwedl, I. et al. Analysis of chromosome-11 aberrations in pulmonary and gastrointestinal carcinoids: an array comparative genomic hybridization-based study. Virchows Arch 445, 151–159 (2004). https://doi.org/10.1007/s00428-004-1052-y

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