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Expression of TpNRAMP5, a metal transporter from Polish wheat (Triticum polonicum L.), enhances the accumulation of Cd, Co and Mn in transgenic Arabidopsis plants

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Abstract

Main conclusion

TpRNAMP5 is mainly expressed in the plasma membrane of roots and basal stems. It functions as a metal transporter for Cd, Mn and Co accumulation.

Numerous natural resistance-associated macrophage proteins (NRAMPs) have been functionally identified in various plant species, including Arabidopsis, rice, soybean and tobacco, but no information is available on NRAMP genes in wheat. In this study, we isolated a TpNRAMP5 from dwarf Polish wheat (DPW, Triticum polonicum L.), a species with high tolerance to Cd and Zn. Expression pattern analysis revealed that TpNRAMP5 is mainly expressed in roots and basal stems of DPW. TpNRAMP5 was localized at the plasma membrane of Arabidopsis leaf protoplast. Expression of TpNRAMP5 in yeast significantly increased yeast sensitivity to Cd and Co, but not Zn, and enhanced Cd and Co concentrations. Expression of TpNRAMP5 in Arabidopsis significantly increased Cd, Co and Mn concentrations in roots, shoots and whole plants, but had no effect on Fe and Zn concentrations. These results indicate that TpNRAMP5 is a metal transporter enhancing the accumulation of Cd, Co and Mn, but not Zn and Fe. Genetic manipulation of TpNRAMP5 can be applied in the future to limit the transfer of Cd from soil to wheat grains, thereby protecting human health.

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Abbreviations

DPW:

Dwarf Polish wheat

IRT:

Iron-regulated transporter

NRAMP:

Natural resistance-associated macrophage protein

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Acknowledgements

The authors thank the National Natural Science Foundation of China (No. 31470305 and 31671688) for all financial support. We thank Barbara Goodson, PhD, from Liwen Bianji, Edanz Group, China (http://www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

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  1. Fan Peng, Chao Wang and Jianshu Zhu contributed equally to this work.

Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China

    Fan Peng, Chao Wang, Jianshu Zhu, Houyang Kang, Xing Fan, Lina Sha, Haiqin Zhang, Yonghong Zhou & Yi Wang

  2. Joint International Research Laboratory of Crop Resources and Genetic Improvement, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China

    Fan Peng, Chao Wang, Jianshu Zhu, Houyang Kang, Xing Fan, Lina Sha, Haiqin Zhang, Yonghong Zhou & Yi Wang

  3. College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China

    Jian Zeng

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  1. Fan Peng
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Correspondence to Yi Wang.

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Peng, F., Wang, C., Zhu, J. et al. Expression of TpNRAMP5, a metal transporter from Polish wheat (Triticum polonicum L.), enhances the accumulation of Cd, Co and Mn in transgenic Arabidopsis plants. Planta 247, 1395–1406 (2018). https://doi.org/10.1007/s00425-018-2872-3

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