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Defense responses in plants of Eucalyptus elicited by Streptomyces and challenged with Botrytis cinerea

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Abstract

Main conclusion

Elicitation of E. grandis plants with Streptomyces PM9 reduced the gray-mold disease, through increasing the levels of enzymes directly related to the induction of plant defense responses, and accumulation of specific phenolic compounds.

Members of Eucalyptus are economically important woody species, especially as a raw material in many industrial sectors. Species of this genus are susceptible to pathogens such as Botrytis cinerea (gray mold). Biological control of plant diseases using rhizobacteria is one alternative to reduce the use of pesticides and pathogen attack. This study evaluated the metabolic and phenotypic responses of Eucalyptus grandis and E. globulus plants treated with Streptomyces sp. PM9 and challenged with the pathogenic fungus B. cinerea. Metabolic responses were evaluated by assessing the activities of the enzymes polyphenol oxidase and peroxidase as well as the levels of phenolic compounds and flavonoids. The incidence and progression of the fungal disease in PM9-treated plants and challenged with B. cinerea were evaluated. Treatment with Streptomyces sp. PM9 and challenge with B. cinerea led to changes in the activities of polyphenol oxidase and peroxidase as well as in the levels of phenolic compounds in the plants at different time points. Alterations in enzymes of PM9-treated plants were related to early defense responses in E. grandis. Gallic and chlorogenic acids were on average more abundant, although caffeic acid, benzoic acid and catechin were induced at specific time points during the culture period. Treatment with Streptomyces sp. PM9 significantly delayed the establishment of gray mold in E. grandis plants. These results demonstrate the action of Streptomyces sp. PM9 in inducing plant responses against B. cinerea, making this organism a potential candidate for biological control in Eucalyptus.

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Abbreviations

AUDPC:

Area under the disease progress curve

dpi:

Days after inoculation

ISR:

Induced systemic resistance

PGPR:

Plant growth promoting rhizobacteria

POX:

Peroxidases

PPO:

Polyphenol oxidases

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Acknowledgments

Authors thank Dr Hans-Peter Fiedler and Dr Rüdiger Hampp from Tübingen University, Germany, for providing the rhizobacteria isolates, to Suzano Papel e Celulose for providing the Eucalyptus seeds and to Janaina Belquis da S. P. Langois for technical assistance. This work was supported by PROBOLSAS-PUCRS (fellowship of first author) and by the National Council for Scientific and Technological Development (CNPq)/Brazil through the Program for Undergraduate Students.

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  1. Laboratory of Plant Biotechnology, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Avenida Ipiranga, 6681, 12C, sala 213, Porto Alegre, RS, 90619-900, Brazil

    Tamiris D. Salla, Leandro V. Astarita & Eliane R. Santarém

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  1. Tamiris D. Salla
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  2. Leandro V. Astarita
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Correspondence to Eliane R. Santarém.

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Salla, T.D., Astarita, L.V. & Santarém, E.R. Defense responses in plants of Eucalyptus elicited by Streptomyces and challenged with Botrytis cinerea . Planta 243, 1055–1070 (2016). https://doi.org/10.1007/s00425-015-2460-8

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