Abstract
Plant viruses cause many diseases that lead to significant economic losses. However, most of the approaches to control plant viruses, including transgenic processes or drugs are plant-species-limited or virus-species-limited, and not very effective. We introduce an application of jasmonic acid (JA) and salicylic acid (SA), a broad-spectrum, efficient and nontransgenic method, to improve plant resistance to RNA viruses. Applying 0.06 mM JA and then 0.1 mM SA 24 h later, enhanced resistance to Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Turnip crinkle virus (TCV) in Arabidopsis, tobacco, tomato and hot pepper. The inhibition efficiency to virus replication usually achieved up to 80–90%. The putative molecular mechanism was investigated. Some possible factors affecting the synergism of JA and SA have been defined, including WRKY53, WRKY70, PDF1.2, MPK4, MPK2, MPK3, MPK5, MPK12, MPK14, MKK1, MKK2, and MKK6. All genes involving in the synergism of JA and SA were investigated. This approach is safe to human beings and environmentally friendly and shows potential as a strong tool for crop protection against plant viruses.
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Abbreviations
- JA:
-
Jasmonic acid
- SA:
-
Salicylic acid
- CMV:
-
Cucumber mosaic virus
- TMV:
-
Tobacco mosaic virus
- TCV:
-
Turnip crinkle virus
- TNV:
-
Tobacco necrosis virus
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Acknowledgments
Arabidopsis thaliana seeds carrying NahG gene were gifts from Prof. Jia Li (University of Oklahoma, USA and Lanzhou University, China). Other Arabidopsis mutants were acquired from Arabidopsis Biological Resource Center (Ohio State University, Columbus, OH, USA). We thank Mr. Rupu Xiao (Certcana Systems Institute, Ontario, Canada) for the language edition. This work was supported by the National Key Basic Research ‘973’ Program of China (2009CB118500), National Nature Science Foundation of China (31070210, 30970214 and 30800071) and Sichuan Nature Science Foundation (2010JQ0080).
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Shang, J., Xi, DH., Xu, F. et al. A broad-spectrum, efficient and nontransgenic approach to control plant viruses by application of salicylic acid and jasmonic acid. Planta 233, 299–308 (2011). https://doi.org/10.1007/s00425-010-1308-5
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DOI: https://doi.org/10.1007/s00425-010-1308-5