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Profiles of the biosynthesis and metabolism of pyridine nucleotides in potatoes (Solanum tuberosum L.)

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Abstract

As part of a research program on nucleotide metabolism in potato tubers (Solanum tuberosum L.), profiles of pyridine (nicotinamide) metabolism were examined based on the in situ metabolic fate of radio-labelled precursors and the in vitro activities of enzymes. In potato tubers, [3H]quinolinic acid, which is an intermediate of de novo pyridine nucleotide synthesis, and [14C]nicotinamide, a catabolite of NAD, were utilised for pyridine nucleotide synthesis. The in situ tracer experiments and in vitro enzyme assays suggest the operation of multiple pyridine nucleotide cycles. In addition to the previously proposed cycle consisting of seven metabolites, we found a new cycle that includes newly discovered nicotinamide riboside deaminase which is also functional in potato tubers. This cycle bypasses nicotinamide and nicotinic acid; it is NAD → nicotinamide mononucleotide → nicotinamide riboside → nicotinic acid riboside → nicotinic acid mononucleotide → nicotinic acid adenine dinucleotide → NAD. Degradation of the pyridine ring was extremely low in potato tubers. Nicotinic acid glucoside is formed from nicotinic acid in potato tubers. Comparative studies of [carboxyl-14C]nicotinic acid metabolism indicate that nicotinic acid is converted to nicotinic acid glucoside in all organs of potato plants. Trigonelline synthesis from [carboxyl-14C]nicotinic acid was also found. Conversion was greater in green parts of plants, such as leaves and stem, than in underground parts of potato plants. Nicotinic acid utilised for the biosynthesis of these conjugates seems to be derived not only from the pyridine nucleotide cycle, but also from the de novo synthesis of nicotinic acid mononucleotide.

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Abbreviations

NaAD:

Nicotinic acid adenine dinucleotide

NaG:

Nicotinic acid N-glucoside

NaMN:

Nicotinic acid mononucleotide

NaR:

Nicotinic acid N-riboside

NMN:

Nicotinamide mononucleotide

NR:

Nicotinamide riboside

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  1. Riko Katahira

    Present address: Department of Lifestyle Studies, Kobe Shoin Women’s University, Hyogo, 657-0015, Japan

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  1. Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, Tokyo, 112-8610, Japan

    Riko Katahira & Hiroshi Ashihara

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  1. Riko Katahira
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  2. Hiroshi Ashihara
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Correspondence to Hiroshi Ashihara.

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Katahira, R., Ashihara, H. Profiles of the biosynthesis and metabolism of pyridine nucleotides in potatoes (Solanum tuberosum L.). Planta 231, 35–45 (2009). https://doi.org/10.1007/s00425-009-1023-2

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