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Stress-induced curcin-L promoter in leaves of Jatropha curcas L. and characterization in transgenic tobacco

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Abstract

Ribosome-inactivating proteins (RIPs) represent a type of protein that universally inactivates the ribosome thus inhibiting protein biosynthesis. Curcin-L was a type I RIP found in Jatropha curcas L.. Its expression could be activated in leaves by treatments with abscisic acid, salicylic acid, polyethylene glycol, temperature 4, 45°C and ultraviolet light. A 654 bp fragment of a 5′ flanking region preceding the curcin-L gene, designated CP2, was cloned from the J. curcas genome and its expression pattern was studied via the expression of the β-glucuronidase (GUS) gene in transgenic tobacco. Analysis of GUS activities showed that the CP2 was leaf specific, and was able to drive the expression of the reporter gene under stress-induction conditions. Analysis of a series of 5′-deletions of the CP2 suggested that several promoter motifs were necessary to respond to environmental stresses.

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Abbreviations

ABA:

Abscisic acid

SA:

Salicylic acid

PEG:

Polyethylene glycol

UV:

Ultraviolet light

GUS:

β-Glucuronidase

MU:

Methylumbelliferone

MUG:

4-Methylumbelliferyl-beta-d-glucuronide

X-gluc:

5-Bromo-4-chloro-3-indolyl-beta-d-glucuronide

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Acknowledgments

This work was supported by the “11th Five-Year Plan” to support science and technology project (No. 2006BAD07A04, No. 2007BAD50B05), General Program of National Natural Science Foundation of China (No. 30670204), China International Science and Technology Cooperation Project (No. 2006DFB63400), and the Key Technology Study Project of Sichuan (No. 05NG002-002-1).

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Authors and Affiliations

  1. College of Life Sciences, Sichuan University, 610064, Chengdu, People’s Republic of China

    Xiaobo Qin, Xiaojiang Zheng, Caixia Shao, Jihai Gao, Luding Jiang, Xunlu Zhu, Fang Yan, Lin Tang, Ying Xu & Fang Chen

  2. International Research Center of Jatropha, Sichuan University, 610064, Chengdu, People’s Republic of China

    Xiaobo Qin, Xiaojiang Zheng, Caixia Shao, Jihai Gao, Xunlu Zhu, Fang Yan, Lin Tang, Ying Xu & Fang Chen

  3. Institute of Materia Medica, College of Pharmacy, Third Military Medical University, 400038, Chongqing, China

    Luding Jiang

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  1. Xiaobo Qin
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Correspondence to Fang Chen.

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Qin, X., Zheng, X., Shao, C. et al. Stress-induced curcin-L promoter in leaves of Jatropha curcas L. and characterization in transgenic tobacco. Planta 230, 387–395 (2009). https://doi.org/10.1007/s00425-009-0956-9

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