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A Ds-insertion mutant of OSH6 (Oryza sativa Homeobox 6) exhibits outgrowth of vestigial leaf-like structures, bracts, in rice

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Abstract

OSH6 (Oryza sativa Homeobox6) is an ortholog of lg3 (Liguleless3) in maize. We generated a novel allele, termed OSH6-Ds, by inserting a defective Ds element into the third exon of OSH6, which resulted in a truncated OSH6 mRNA. The truncated mRNA was expressed ectopically in leaf tissues and encoded the N-terminal region of OSH6, which includes the KNOX1 and partial KNOX2 subdomains. This recessive mutant showed outgrowth of bracts or produced leaves at the basal node of the panicle. These phenotypes distinguished it from the OSH6 transgene whose ectopic expression led to a “blade to sheath transformation” phenotype at the midrib region of leaves, similar to that seen in dominant Lg3 mutants. Expression of a similar truncated OSH6 cDNA from the 35S promoter (35S::ΔOSH6) confirmed that the ectopic expression of this product was responsible for the aberrant bract development. These data suggest that OSH6-Ds interferes with a developmental mechanism involved in bract differentiation, especially at the basal nodes of panicles.

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Abbreviations

kn1:

Knotted1

KNOX:

KNOTTED1-like homeobox

lg3:

Liguleless3

OSH6:

Oryza sativa Homeobox6

TALE HD:

Three amino acid loop extension homeodomain

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Acknowledgments

This work was supported by a grant (code # CG151) from Crop Functional Genomics Center of the 21st Century Frontier Research Program. This work also was supported by Korea Research Foundation Grant (KRF-2003-015-C00636), BioGreen 21 Program (Rural Development Administration), by Basic Research Fund (NIAB, 06-2-12-7-1), and from the KOSEF/MOST to the Environmental Biotechnology National Core Research Center (R15-2003-012-01001-0). Sung Han Park, Byoung Il Je, Soon Ju Park, and Hai Long Piao are recipients of fellowships of the Brain Korea21 project. We appreciate very much the comments of Dr. David Jackson at the Cold Spring Harbor Lab (USA).

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  1. Mi Sook Choe

    Present address: , 261 Congressional in # 418, Rockville, MD, 20852, USA

Authors and Affiliations

  1. Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, South Korea

    Sung Han Park, Chul Min Kim, Byoung Il Je, Su Hyun Park, Soon Ju Park, Hai Long Piao, Yuan-hu Xuan, Mi Sook Choe, Kon Ho Lee & Chang-deok Han

  2. Department of Molecular Genetics, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602, Japan

    Kouji Satoh & Shoshi Kikuchi

  3. Rice Functional Genomics, National Institute of Agricultural Biotechnology, RDA, Suwon, 441-707, South Korea

    Sung Han Park, Young Soon Cha, Byung Ohg Ahn, Hyeon So Ji, Doh Won Yun, Myung Chul Lee, Seok-cheol Suh & Moo Young Eun

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Correspondence to Moo Young Eun or Chang-deok Han.

Electronic supplementary material

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Supplementary Fig. 1 Comparison of

OSH6-Ds mRNA levels between mutants (m/m) and ones (OSH6, m/m) expressing 35S::OSH6 by RT-PCR. RT-PCR analysis was performed to detect the mRNAs of OSH6-Ds (m/m) and 35S::OSH6 (OSH6) in plants of single and double genetic combinations. OSH6-Ds transcripts were examined by RT-PCR using the primer pair 3-4/5-2 shown in Fig. 4a. OSH6 mRNA from 35S::OSH6 was detected using a primer pair 3F/3R shown in Fig. 3a. The locations of these primer sets are shown in Fig. 3a. Actin mRNA was used as a control (TIFF 970 kB)

Supplementary Fig. 2 Phenotypic comparisons of panicle nodes in mutant plants (m/m) expressing

OSH6-Ds and in plants (OSH6, m/m) expressing 35S-OSH6 and OSH6-Ds. Pancle nodes are shown in mature plants of wild type, OSH6-Ds, 35S-OSH6, and OSH-Ds/3 5S-OSH6 plants. Arrows indicate ‘bract-leaf’ (TIFF 3.51 MB)

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Park, S.H., Kim, C.M., Je, B.I. et al. A Ds-insertion mutant of OSH6 (Oryza sativa Homeobox 6) exhibits outgrowth of vestigial leaf-like structures, bracts, in rice. Planta 227, 1–12 (2007). https://doi.org/10.1007/s00425-007-0576-1

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