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Mutation in a homolog of yeast Vps53p accounts for the heat and osmotic hypersensitive phenotypes in Arabidopsis hit1-1 mutant

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Abstract

Previously, the growth of Arabidopsis hit1-1 (heat-intolerant) mutant was found to be inhibited by both heat and water stress (Wu et al. in J Plant Physiol 157:543–547, 2000). In order to determine the genetic mutation underlying the hit1-1 phenotype, map-based cloning of HIT1 gene was conducted. Transformation of the hit1-1 mutant with a HIT1 cDNA clone reverts the mutant to the heat tolerance phenotype, confirming the identity of HIT1. Sequence analysis revealed the HIT1 gene encodes a protein of 829 amino acid residues and is homologous to yeast (Saccharomyces cerevisiae) Vps53p protein. The yeast Vps53p protein has been shown to be a tethering factor that associates with Vps52p and Vps54p in a complex formation involved in the retrograde trafficking of vesicles to the late Golgi. An Arabidopsis homolog of yeast Vps52p has previously been identified and mutation of either the homolog or HIT1 by T-DNA insertion resulted in a male-specific transmission defect. The growth of yeast vps53Δ null mutant also shows reduced thermotolerance, and expression of HIT1 in this mutant can partially complement the defect, supporting the possibility of a conserved biological function for Vps53p and HIT1. Collectively, the hit1-1 is the first mutant in higher plant linking a homolog of the vesicle tethering factor to both heat and osmotic stress tolerance.

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Abbreviations

SNARE:

Soluble N-ethylmaleimide-sensitive factor adaptor protein receptor

SSLP:

Simple sequence length polymorphism

CAPS:

Cleaved amplified polymorphic sequence

SNP:

Single nucleotide polymorphism

HSP:

Heat shock protein

LEA:

Late embryogenesis abundant

COR:

Cold regulated

VPS:

Vesicular protein sorting

RT-PCR:

Reverse transcription-polymerase chain reaction

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Acknowledgement

This work was supported by National Science Council (Taiwan) grants NSC 92-2311-B-008-009 and NSC 94-2311-B-008-003 (S.-J. Wu). We thank Ms. Kuei-Yun Chang for her excellent sequencing works. Help from Drs. Kuo-Chen Yeh (Institute of BioAgricultural Sciences, Academia Sinica, Taiwan), Shu-Hsing Wu (Institute of Plant and Microbial Biology, Academia Sinica, Taiwan), and Chung-An Lu (Department of Biological Sciences, National Central University, Taiwan) is appreciated. The spiritual support from Ms. Shu-Sheng Chang is also acknowledged.

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Authors and Affiliations

  1. Department of Life Sciences, National Central University, 300 Jhong-da Road, 320, Jhong-li City, Taoyuan, Taiwan

    Chai-Fong Lee, Hsin-Yi Pu, Lian-Chin Wang, Ching-Hui Yeh & Shaw-Jye Wu

  2. Department of Plant Pathology, University of Arkansas, 217 Plant Science, Fayetteville, AR, 72701, USA

    Ronald J. Sayler

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  1. Chai-Fong Lee
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Correspondence to Shaw-Jye Wu.

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Lee, CF., Pu, HY., Wang, LC. et al. Mutation in a homolog of yeast Vps53p accounts for the heat and osmotic hypersensitive phenotypes in Arabidopsis hit1-1 mutant. Planta 224, 330–338 (2006). https://doi.org/10.1007/s00425-005-0216-6

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