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Abscisic acid-inducible nuclear proteins bind to bipartite promoter elements required for ABA response and embryo-regulated expression of the carrot Dc3 gene

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Abstract

The carrot (Daucus carota L.) lea-class gene Dc3 is expressed in developing seeds and in vegetative tissues subject to drought and treatment with exogenous abscisic acid (ABA). Cis regulatory elements involved in seed-specific expression and in response to ABA were identified in transgenic tobacco (Nicotiana tabacum L.) using β-glucuronidase (GUS) reporter gene constructs containing a series of deletion and orientation mutants of the Dc3 promoter. These experiments demonstrated that the Dc3 promoter is comprised of a proximal promoter region (PPR) and a distal promoter region (DPR). TCGTGT motifs in the DPR in combination with the PPR comprise a novel, bipartite ABA module in the Dc3 gene promoter. The PPR contains cis-acting elements responsible for the developmental regulation of Dc3 expression in seeds. Five similar sequence motifs with the consensus ACACgtGCa were identified in the PPR. Both DPR and PPR interact with common nuclear proteins that are present in embryos and are inducible by ABA in vegetative tissues.

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Abbreviations

ABA :

Abscisic acid

ABRE :

ABA-response element

bZIP :

Basic leucine zipper

DAR :

Distal ABA-response region

DPBF :

Dc3 promoter-binding factor

DPR :

Distal promoter region

GMSA :

Gel mobility-shift assay

GUS :

β-Glucuronidase

LEA :

Late embryogenesis abundant

PPR :

Proximal promoter region

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Acknowledgement

This work was financially supported in part by USDA NRICGP grants (Nos. 94373041228, 97353044552, and 013530410940).

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Authors and Affiliations

  1. Department of Biology, Texas A&M University, College Station, TX 77843-3258, USA

    Terry L. Thomas

  2. Laboratory of Functional Genomics for Plant Secondary Metabolism, Eugentech Inc., 305-333, Daejon, Korea

    Hwa-Jee Chung

  3. Institute of Botany, Academia Sinica, Nankang, 11529, Taipei, Taiwan, R.O.C.

    Hong Yong Fu

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  1. Hwa-Jee Chung
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Correspondence to Terry L. Thomas.

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Chung, HJ., Fu, H.Y. & Thomas, T.L. Abscisic acid-inducible nuclear proteins bind to bipartite promoter elements required for ABA response and embryo-regulated expression of the carrot Dc3 gene. Planta 220, 424–433 (2005). https://doi.org/10.1007/s00425-004-1366-7

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