Abstract
Previous genetic analysis identified a component, RED1, that is required for normal de-etiolation of Arabidopsis thaliana (L.) Heynh. seedlings in continuous red light (Rc). red1 mutant seedlings exhibit elongated hypocotyls and reduced cotyledon size specifically in Rc and not in continuous far-red light (FRc). Here, we show that red1 is allelic to sur2 and atr4, and is defective in the cytochrome P450 CYP83B1, an enzyme required for normal auxin homeostasis. Two alleles of atr4, like red1, exhibit increased hypocotyl elongation and reduced cotyledon expansion in Rc but not in FRc. We further show that CYP83B1 transcript levels are elevated specifically in Rc-grown seedlings when compared with seedlings grown in darkness or FRc. Hence, the Rc-specific phenotype of the red1 mutant may indicate that Rc-induction of the CYP83B1 transcript is necessary for normal seedling de-etiolation in the wild type.
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Abbreviations
- FR :
-
Far-red light
- FRc :
-
Continuous FR
- IAA :
-
Indole-3-acetic acid
- IAOx :
-
Indole-3-acetaldoxime
- phyA :
-
Phytochrome A
- phyB :
-
Phytochrome B
- R :
-
Red light
- Rc :
-
Continuous R
References
Bak S, Tax FE, Feldmann KA, Galbraith DW, Feyereisen R (2001) CYP83B1, a cytochrome P450 at the metabolic branch point in auxin and indole glucosinolate biosynthesis in Arabidopsis. Plant Cell 13:101–111
Barlier I, Kowalczyk M, Marchant A, Ljung K, Bhalerao R, Bennett M, Sandberg G, Bellini C (2000) The SUR2 gene of Arabidopsis thaliana encodes the cytochrome P450 CYP83B1, a modulator of auxin homeostasis. Proc Natl Acad Sci USA 97:14819–14824
Behringer FJ, Davies PJ (1992) Indole-3-acetic acid levels after phytochrome-mediated changes in the stem elongation rate of dark- and light-grown Pisum seedlings. Planta 188:85–92
Boerjan W, Cervera MT, Delarue M, Beeckman T, Dewitte W, Bellini C, Caboche M, Van Onckelen H, Van Montagu M, Inze D (1995) superroot, a recessive mutation in Arabidopsis, confers auxin overproduction. Plant Cell 7:1405–1419
Delarue M, Prinsen E, Onckelen HV, Caboche M, Bellini C (1998) Sur2 mutations of Arabidopsis thaliana define a new locus involved in the control of auxin homeostasis. Plant J 14:603–611
Fankhauser C, Staiger D (2002) Photoreceptors in Arabidopsis thaliana: light perception, signal transduction and entrainment of the endogenous clock. Planta 216:1–16
Friml J, Palme K (2002) Polar auxin transport—old questions and new concepts? Plant Mol Biol 49:273–284
Gray WM, Ostin A, Sandberg G, Romano CP, Estelle M (1998) High temperature promotes auxin-mediated hypocotyl elongation in Arabidopsis. Proc Natl Acad Sci USA 95:7197–7202
Halliday KJ, Fankhauser C (2003) Phytochrome-hormonal signalling networks. New Phytol 157:449–463
Hansen CH, Du L, Naur P, Olsen CE, Axelsen KB, Hick AJ, Pickett JA, Halkier BA (2001) CYP83b1 is the oxime-metabolizing enzyme in the glucosinolate pathway in Arabidopsis. J Biol Chem 276:24790–24796
Hemm MR, Ruegger MO, Chapple C (2003) The Arabidopsis ref2 mutant is defective in the gene encoding CYP83A1 and shows both phenylpropanoid and glucosinolate phenotypes. Plant Cell 15:179–194
Hoecker U, Quail PH (2001) The phytochrome A-specific signaling intermediate SPA1 interacts directly with COP1, a constitutive repressor of light signaling in Arabidopsis. J Biol Chem 276:38173–38178
Hoecker U, Xu Y, Quail PH (1998) SPA1: a new genetic locus involved in phytochrome A-specific signal transduction. Plant Cell 10:19–33
Jensen PJ, Hangarter RP, Estelle M (1998) Auxin transport is required for hypocotyl elongation in light-grown but not dark-grown Arabidopsis. Plant Physiol 116:455–462
Jones AM, Cochran DS, Lamerson PM, Evans ML, Cohen JD (1991) Red light-regulated growth. I. Changes in the abundance of indoleacetic acid and a 22-kilodalton auxin-binding protein in the maize mesocotyl. Plant Physiol 97:352–358
Koornneef M, Rolff E, Spruit CJP (1980) Genetic control of light-inhibited hypocotyl elongation in Arabidopsis thaliana. Z Pflanzenphysiol 100:147–160
Kraepiel Y, Agnes C, Thiery L, Maldiney R, Miginiac E, Delarue M (2001) The growth of tomato (Lycopersicon esculentum Mill.) hypocotyls in the light and in darkness differentially involves auxin. Plant Sci 161:1067–1074
Nagy F, Schäfer E (2002) Phytochromes control photomorphogenesis by differentially regulated, interacting signaling pathways in higher plants. Annu Rev Plant Biol 53:329–355
Neff MM, Fankhauser C, Chory J (2000) Light: an indicator of time and place. Genes Dev 14:257–271
Quail PH (2002) Phytochrome photosensory signalling networks. Nat Rev Mol Cell Biol 3:85–93
Romano CP, Robson PR, Smith H, Estelle M, Klee H (1995) Transgene-mediated auxin overproduction in Arabidopsis: hypocotyl elongation phenotype and interactions with the hy6-1 hypocotyl elongation and axr1 auxin-resistant mutants. Plant Mol Biol 27:1071–1083
Saibo NJ, Vriezen WH, Beemster GT, Van Der Straeten D (2003) Growth and stomata development of Arabidopsis hypocotyls are controlled by gibberellins and modulated by ethylene and auxins. Plant J 33:989–1000
Smalle J, Haegman M, Kurepa J, Van Montagu M, Straeten DV (1997) Ethylene can stimulate Arabidopsis hypocotyl elongation in the light. Proc Natl Acad Sci USA 94:2756–2761
Smolen G, Bender J (2002) Arabidopsis cytochrome P450 cyp83B1 mutations activate the tryptophan biosynthetic pathway. Genetics 160:323–332
Swarup R, Parry G, Graham N, Allen T, Bennett M (2002) Auxin cross-talk: integration of signalling pathways to control plant development. Plant Mol Biol 49:411–426
Wagner D, Tepperman JM, Quail PH (1991) Overexpression of phytochrome B induces a short hypocotyl phenotype in transgenic Arabidopsis. Plant Cell 3:1275–11288
Wagner D, Hoecker U, Quail PH (1997) RED1 is necessary for phytochrome B-mediated red light-specific signal transduction in Arabidopsis. Plant Cell 9:731–743
Zhao Y, Christensen SK, Fankhauser C, Cashman JR, Cohen JD, Weigel D, Chory J (2001) A role for flavin monooxygenase-like enzymes in auxin biosynthesis. Science 291:306–309
Acknowledgements
We are grateful to Sharon Moran for excellent technical assistance. We thank Anastasios Theologis for critical reading of the manuscript. This research was supported by DOE grant DE-FG03-87ER13742 and USDA-ARS CRIS 5335-21000-017-00D. G.T.O. was the recipient of a fellowship from the Consejo Nacional de Ciencia y Tecnológia Fulbright and the University of California-Mexus.
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Hoecker, U., Toledo-Ortiz, G., Bender, J. et al. The photomorphogenesis-related mutant red1 is defective in CYP83B1, a red light-induced gene encoding a cytochrome P450 required for normal auxin homeostasis. Planta 219, 195–200 (2004). https://doi.org/10.1007/s00425-004-1211-z
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DOI: https://doi.org/10.1007/s00425-004-1211-z