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Recovery of photosystem I and II activities during re-hydration of lichen Hypogymnia physodes thalli

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Abstract

Photochemical efficiencies of photosystem I (PSI) and photosystem II (PSII) were studied in dry thalli of the lichen Hypogymnia physodes and during their re-hydration. In dry thalli, PSII reaction centers are photochemically inactive, as evidenced by the absence of variable chlorophyll (Chl) fluorescence, whereas the primary electron donor of PSI, P700, exhibits irreversible oxidation under continuous light. Upon application of multiple- and, particularly, single-turnover pulses in dry lichen, P700 oxidation partially reversed, which indicated recombination between P700+ and the reduced acceptor FX of PSI. Re-wetting of air-dried H. physodes initiated the gradual restoration of reversible light-induced redox reactions in both PSII and PSI, but the recovery was faster in PSI. Two slow components of P700+ reduction occurred after irradiation of partially and completely hydrated thalli with strong white light. In contrast, no slow component was found in the kinetics of re-oxidation of QA −, the reduced primary acceptor of PSII, after exposure of such thalli to white light. This finding indicated the inability of PSII in H. physodes to provide the reduction of the plastoquinone pool to significant levels. It is concluded that slow alternative electron transport routes may contribute to the energetics of photosynthesis to a larger extent in H. physodes than in higher plants.

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Fig. 1
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Fig. 8
Fig. 9a,b

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Abbreviations

A 0 and A 1 :

Primary acceptor chlorophyll and secondary electron acceptor phylloquinone

Chl a :

Chlorophyll a

F m :

Maximal level of chlorophyll fluorescence when all PSII centers are closed

F o :

Minimal level of fluorescence when all PSII centers are open after dark adaptation

FR :

Far-red

F v :

Variable fluorescence (=F m−F o)

F X , F A , and F B :

Iron–sulfur centers

MT pulse :

Multiple-turnover pulse

PS :

Photosystem

P700 :

Reaction center chlorophyll of PSI

Q A :

Primary quinone acceptor of PSII

Q B :

Secondary quinone acceptor of PSII

ST pulse :

Single-turnover pulse

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Acknowledgements

The authors thank Dr. G. Brudvig for helpful discussion of this work and J. Harnois for kind professional assistance. This research was initiated in the laboratory of Dr. U. Heber, University of Würzburg, Germany. N.B. received some support from the Deutsche Forschungsgemeinschaft. Financial support from the Natural Sciences and Engineering Research Council of Canada is also acknowledged.

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Authors and Affiliations

  1. Groupe de Recherche en Énergie et Information Biomoléculaires, Université du Québec à Trois-Rivières, Québec, G9A 5H7, Canada

    Nikolai G. Bukhov, Sridharan Govindachary, Elena A. Egorova & Robert Carpentier

  2. K.A. Timiriazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia

    Nikolai G. Bukhov & Elena A. Egorova

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  1. Nikolai G. Bukhov
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  2. Sridharan Govindachary
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  3. Elena A. Egorova
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  4. Robert Carpentier
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Correspondence to Robert Carpentier.

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Bukhov, N.G., Govindachary, S., Egorova, E.A. et al. Recovery of photosystem I and II activities during re-hydration of lichen Hypogymnia physodes thalli. Planta 219, 110–120 (2004). https://doi.org/10.1007/s00425-003-1195-0

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