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Revisiting TRPC1 and TRPC6 mechanosensitivity

  • Ion Channels
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Abstract

This article addresses whether TRPC1 or TRPC6 is an essential component of a mammalian stretch-activated mechano-sensitive Ca2+ permeable cation channel (MscCa). We have transiently expressed TRPC1 and TRPC6 in African green monkey kidney (COS) or Chinese hamster ovary (CHO) cells and monitored the activity of the stretch-activated channels using a fast pressure clamp system. Although both TRPC1 and TRPC6 are highly expressed at the protein level, the amplitude of the mechano-sensitive current is not significantly altered by overexpression of these subunits. In conclusion, although several TRPC channel members, including TRPC1 and TRPC6, have been recently proposed to form MscCa in vertebrate cells, the functional expression of these TRPC subunits in heterologous systems remains problematic.

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Acknowledgements

JF, DB, AP, and EH are grateful to the ANR 2005 Cardiovasculaire-obésité-diabète; to the Association for Information and Research on Genetic Kidney Disease, France; to the Fondation del Duca; to the Fondation de France; to the Fondation de la Recherche Médicale; to EU Marie-Curie fellowships, to INSERM; to Human Frontier Science program; and to Centre National de la Recherche Scientifique for support. RM and OH thank the Department of Defense Prostate Cancer Research Program and the National Cancer Institute for their support. The Buffalo group was supported by the NIH and the Oshei Foundation. AR and BM thank the Australian Research Council for support. We are grateful to Wade J. Sigurdson, Director of the Confocal Microscope, and three-dimensional Imaging Facility at UB, and Leoncio Vergara of the Optical Imaging Laboratory (UTMB) for help with imaging.

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Authors and Affiliations

  1. Single Molecule Biophysics, SUNY, 301 Cary Hall, Buffalo, NY, 14214, USA

    Philip Gottlieb, Charles Bowman & Frederick Sachs

  2. IPMC-CNRS, UMR 6097, Université de Nice-Sophia Antipolis, 06560, Valbonne, France

    Joost Folgering, Delphine Bichet, Amanda Patel & Eric Honoré

  3. Department of Neuroscience & Cell Biology, UTMB, Galveston, TX, 77555, USA

    Rosario Maroto & Owen P. Hamill

  4. School of Medicine and Pharmacology, University of Western Australia, Crawley, Western Australia, 6009, Australia

    Albert Raso

  5. Department of Biochemistry and Molecular Biology, UTMB, Galveston, TX, 77555, USA

    Thomas G. Wood & Alex Kurosky

  6. School of Biomedical Sciences, University of Queensland, St Lucia, Brisbane, Queensland, 4072, Australia

    Boris Martinac

Authors
  1. Philip Gottlieb
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  2. Joost Folgering
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  10. Frederick Sachs
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Corresponding author

Correspondence to Owen P. Hamill.

Additional information

Co-first authors: Philip Gottlieb, Joost Folgering, and Rosario Maroto.

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Gottlieb, P., Folgering, J., Maroto, R. et al. Revisiting TRPC1 and TRPC6 mechanosensitivity. Pflugers Arch - Eur J Physiol 455, 1097–1103 (2008). https://doi.org/10.1007/s00424-007-0359-3

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  • DOI: https://doi.org/10.1007/s00424-007-0359-3

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