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Interactions of neutrophils with silver-coated vascular polyester grafts

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Abstract

Background

In reconstructive vascular surgery, infection is one of the most feared complications because of the high mortality. While the antimicrobial effect of a silver-coated endoprosthesis has been proven in experimental trials, there are no reports on its interactions with granulocytes, the first effector cells in general inflammation and in infection.

Materials and methods

Therefore, we investigated whether silver coating of vascular polyester grafts affects receptor expression, mediator release, and functions of human neutrophils relevant for microbicidal activity and the wound-healing process. Naïve neutrophils were analyzed for their cellular receptors such as cluster of differentiation (CD)62L, CD11b, CXCR2, and fMLP-R, the mediators interleukin 8, granulocyte elastase (human neutrophil elastase), and leukotriene B4 (LTB4) as well as for microbicidal capacity (oxidative burst) in vitro. In addition, the role of plasma coating for receptor expression was addressed.

Results

There was both a decrease of CD62L and CXCR2 expression and an increase of CD11b, fMLP-R expression, elastase release, and LTB4 generation, which were statistically significant (p = 0.04; p = 0.01; p = 0.0; p = 0.0; p = 0.01; p = 0.02, respectively) in the presence of the silver-coated graft compared with non-silver-coated vascular grafts. In addition, microbicidal activity was significantly (p = 0.0) impaired by the silver-coated graft. Coating of the vascular grafts with plasma did not alter the former observations significantly.

Conclusion

The results may indicate that silver-coated vascular polyester grafts activate neutrophils chronically which may favor tissue destruction and impaired antimicrobial effects.

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Contribution

J.T. performed the experiments; U.S. analyzed results and made the statistics; W.K., B.K., T.B., and H.L. designed the research; J.T., F.M., and B.K. wrote the manuscript.

Conflict of interest disclosure

The authors declare no competing financial interests.

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Authors and Affiliations

  1. Department of General, Abdominal, and Vascular Surgery, University Hospital, Leipziger Strasse 44, 39120, Magdeburg, Germany

    Joerg Tautenhahn, Frank Meyer, Thomas Buerger & Hans Lippert

  2. Institute of Biostatistics, University Hospital, Magdeburg, Germany

    Uwe Schmidt

  3. Institute of Medical Microbiology, University Hospital, Magdeburg, Germany

    Wolfgang Koenig & Brigitte Koenig

Authors
  1. Joerg Tautenhahn
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  2. Frank Meyer
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  3. Thomas Buerger
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  4. Uwe Schmidt
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  5. Hans Lippert
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  6. Wolfgang Koenig
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  7. Brigitte Koenig
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Correspondence to Joerg Tautenhahn.

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Tautenhahn, J., Meyer, F., Buerger, T. et al. Interactions of neutrophils with silver-coated vascular polyester grafts. Langenbecks Arch Surg 395, 143–149 (2010). https://doi.org/10.1007/s00423-008-0439-7

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  • DOI: https://doi.org/10.1007/s00423-008-0439-7

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