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Acute effects of sex-specific sex hormones on heat shock proteins in fast muscle of male and female rats

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Abstract

Heat shock protein (HSP) expression and sex hormone levels have been shown to influence several aspects of skeletal muscle physiology (e.g., hypertrophy, resistance to oxidative stress), suggesting that sex hormone levels can effect HSP expression. This study evaluated the effects of differing levels of sex-specific sex hormones (i.e., testosterone in males and estrogen in females) on the expression of 4: HSP70, HSC70, HSP25, and αB-crystallin in the quadriceps muscles of male and female rats. Animals were assigned to 1 of 3 groups (n = 5 M and F/group). The first group (Ctl) consisted of typically cage-housed animals that served as controls. The second group (H) was gonadectomized and received either testosterone (males) or estradiol (females) via injection for 12 consecutive days. The third group (Gx) was gonadectomized and injected as above, but with vehicle only, rather than hormones. Significant sex by condition interactions (P < 0.05 by two-way MANOVA) were found for all 4 proteins studied, except for HSP70, which exhibited a significant effect of condition only. The expression of all HSPs was greater (1.9–2.5-fold) in males vs. females in the Ctl group, except for HSP70, which was no different. Generally, gonadectomy appeared to have greater effects in males than females, but administration of the exogenous sex hormones tended to produce more robust relative changes in females than males. There were no differences in myosin composition in any of the groups, suggesting that changes in fiber type were not a factor in the differential protein expression. These data may have implications for sex-related differences in muscular responses to exercise, disuse, and injury.

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  1. William A. Romani

    Present address: AARP Experience Corps, 2601 N. Howard St, Baltimore, MD, 21218, USA

Authors and Affiliations

  1. Department of Physical Therapy and Rehabilitation Science, University of Maryland School of Medicine, Baltimore, MD, USA

    William A. Romani

  2. Division of Physical Therapy, School Rehabilitation and Communication Sciences, Ohio University, W290 Grover Center, Rm. W 279, Athens, OH, 45701, USA

    David W. Russ

  3. Ohio Musculoskeletal and Neurological Institute (OMNI), Heritage College of Osteopathic Medicine, Athens, OH, USA

    David W. Russ

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Correspondence to David W. Russ.

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Communicated by William J. Kraemer.

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Romani, W.A., Russ, D.W. Acute effects of sex-specific sex hormones on heat shock proteins in fast muscle of male and female rats. Eur J Appl Physiol 113, 2503–2510 (2013). https://doi.org/10.1007/s00421-013-2686-8

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  • DOI: https://doi.org/10.1007/s00421-013-2686-8

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