Abstract
Studies on gene–phenotype associations are a popular theme in exercise physiology. This editorial follows up on the current limitations in this quest with regard to the identification of mechanistically important relationships.
Following the introduction of and improvement in genomic technologies, there is a rapidly increasing interest in possible genetic factors that may explain differences in physical exercise performance (Bray et al. 2009). During the evaluation of manuscript submissions to the European Journal of Applied Physiology (EJAP), it has become evident that a sizeable proportion of submissions on the topic of human performance now focuses on the influence of genes (Fig. 1). This is illustrated by a series of recent papers (Zago et al. 2010; He et al. 2010; Kostek et al. 2010), including the work of Gabriel Rodríguez-Romo and colleagues published in this issue (Rodríguez-Romo et al. 2010). The results of this latter study show that neither of single polymorphisms in two candidates of muscle phenotypic control, angiotensin converting enzyme (ACE) and α-actinin-3 (ACTN3), is associated with explosive muscle performance in young healthy adults. Given the thousands of possible gene polymorphisms and the rapidly increasing number of studies in this field, there would seem to be a perceived need for guidance to authors who are considering such submissions to EJAP.
An historical perspective appears important to bear in mind when considering the physiological significance of studies designed to map the role of genetic factors in exercise performance. The genetical approach is rooted in quantitative genetics which links gene markers with quantitative traits (Plomin et al. 2009). The usage of such methodology to resolve the mechanisms that regulate the exercise phenotype relates back to the classical work of Petit and Klissouras in twins (Klissouras et al. 1973). Their investigation laid out the discrete contribution of heritable elements which manifest in aerobic capacity. However, early studies on twins showed that environmental factors may be more important than genetic influences in dictating physical performance (Howald et al. 1976). It is now evident that both genetical and environmental cues influence the expression of an endurance/strength exercise phenotype, to a similar degree (Dempfle et al. 2008). Nevertheless, with the sophistication of molecular biological technology (for the sensitive detection of gene polymorphisms via polymerase chain reaction techniques), the genetical approach has gained further popularity (Ahmetov and Rogozkin 2009). It is then hoped that this resulting knowledge can serve, for example, to tailor exercise treatments to at risk populations and also to further mechanistic understanding of the biological pathways that underlie physical performance (Plomin et al. 2009).
There is however an increasing list of examples for which the effects of gene polymorphisms on selected physiological endpoints of performance are either small or lacking. These contrast with the effects that arise from full loss or gain in function mutation, which are described for certain pathologies or transgenic animals (MacArthur et al. 2008). The small effect, coupled with the controversy surrounding the functional consequences of gene polymorphisms, has shed doubts on the relevance of the genetic underpinning of athletic performance. The literature suggests that this disparity on the functional outcome of single gene polymorphisms is a consequence of the multi-factorial nature of biological pathways. With this in mind, success in probing whether a single polymorphism has an important effect on a complex trait is limited to factors with a central role in biological regulation. In most cases, the single-gene approach is likely to reduce the effect size for two reasons. Firstly, the studied factor may not be at the “bottleneck” of regulation. Secondly, the affected event may contribute only marginally to the physiological function at the whole-body level. The first factor is explained by the fact that overlapping signalling pathways are a hallmark for the robustness of biological regulation (Colobran et al. 2007). The second factor is explained, for instance, by many different tissues sharing the control over aerobic performance (Darveau et al. 2002).
Gene polymorphisms, which affect only one regulatory (tissue) component, can be important at the local level. However, the effect may be lost when assessed at the system level. This is highlighted, for instance, by the often-debated question as to whether the heart or skeletal muscle dictates trainability of maximum oxygen uptake (\( \dot{V}\hbox{O}_{2} \)max) (Hoppeler and Lindstedt 2006): the answer appears to be that both contribute to differing degrees (Darveau et al. 2002). Thus, the physiologically assessed endpoint may be too remote to detect the effect of a genetic modification.
The detection of the effect of a single polymorphism on a phenotype is sometimes also complicated by population differences. This can be illustrated by studies of Ins/Del (I/D) polymorphism of the ACE gene. Early studies showed an association between the ACE D allele and better performance in strength-oriented power exercise compared to endurance exercise (see refs in Rodríguez-Romo et al. 2010). However, subsequent studies have revealed a more complex picture (Table 1). Thus, there are differences between different ethnic groups, between trained and untrained subjects etc.
It follows from the above considerations that the implication of a molecular mechanism in the regulation of a phenotypic trait may not be exposed through measuring the relationship between a single point at the bottom (‘the gene’) and the top of a physiological pathway (e.g. function as assessed by \( \dot{V}\hbox{O}_{2} \)max or sprint performance). Thus, we suggest that future studies using a genomic paradigm to expose molecular regulation should employ a more mechanistic approach and focus on functions that are more directly related to the molecular alteration under investigation. This call for a careful test of functional relevance for gene–phenotype associations is in line with suggestions voiced regarding the molecular mechanism of metabolic disease (Loos 2009). We suggest that future studies of whether or how a single gene polymorphism may affect \( \dot{V}\hbox{O}_{2} \)max or sprint performance in different populations will most likely not advance our understanding of the underlying mechanisms that determine performance during physical exercise.
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Communicated by Susan Ward.
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Flueck, M., Vaughan, D. & Westerblad, H. Linking genes with exercise: where is the cut-off?. Eur J Appl Physiol 110, 1095–1098 (2010). https://doi.org/10.1007/s00421-010-1662-9
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DOI: https://doi.org/10.1007/s00421-010-1662-9