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Plasma glucose, insulin and catecholamine responses to a Wingate test in physically active women and men

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Abstract

The influence of gender on the glucose response to exercise remains contradictory. Moreover, to our knowledge, the glucoregulatory responses to anaerobic sprint exercise have only been studied in male subjects. Hence, the aim of the present study was to compare glucoregulatory metabolic (glucose and lactate) and hormonal (insulin, catecholamines and estradiol only in women) responses to a 30-s Wingate test, in physically active students. Eight women [19.8 (0.7) years] and eight men [22.0 (0.6) years] participated in a 30-s Wingate test on a bicycle ergometer. Plasma glucose, insulin, and catecholamine concentrations were determined at rest, at the end of both the warm-up and the exercise period and during the recovery (5, 10, 20, and 30 min). Results showed that the plasma glucose increase in response to a 30-s Wingate test was significantly higher in women than in men [0.99 (0.15) versus 0.33 (0.20) mmol l−1 respectively, P<0.05]. Plasma insulin concentrations peaked at 10 min post-exercise and the increase between this time of recovery and the end of the warm-up was also significantly higher in women than in men [14.7 (2.9) versus 2.3 (1.9) pmol l−1 respectively, P<0.05]. However, there was no gender difference concerning the catecholamine response. The study indicates a gender-related difference in post-exercise plasma glucose and insulin responses after a supramaximal exercise.

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References

  • Astrand I (1960) Work capacity in men and women with a special reference to age. Acta Physiologica Scand S149

  • Bosco C, Tihanyl J, Rivalta L, Parlato G, Tranquilli C, Pulvirenti G, Foti C, Viru M, Viru A (1996) Hormonal responses in strenuous jumping effort. Jpn J Physiol 46(1):93–98

    CAS  PubMed  Google Scholar 

  • Brooks S, Nevill ME, Meleagros L, Lakomy HK, Hall GM, Bloom SR, Williams C (1990) The hormonal responses to repetitive brief maximal exercise in humans. Eur J Appl Physiol 60(2):144–148

    CAS  Google Scholar 

  • Carter SL, Rennie C, Tarnopolsky MA (2001) Substrate utilization during endurance exercise in men and women after endurance training. Am J Physiol 280(6):E898–E907

    CAS  Google Scholar 

  • Coderre L, Kandror KV, Vallega G, Pilch PF (1995) Identification and characterization of an exercise-sensitive pool of glucose transporters in skeletal muscle. J Biol Chem 270(46):27584–27588

    Article  CAS  PubMed  Google Scholar 

  • Coker RH, Krishna MG, Lacy DB, Bracy DP, Wasserman DH (1997a) Role of hepatic alpha- and beta-adrenergic receptor stimulation on hepatic glucose production during heavy exercise. Am J Physiol 273(5 Pt 1):E831–E838

    CAS  PubMed  Google Scholar 

  • Coker RH, Krishna MG, Lacy DB, Allen EJ, Wasserman DH (1997b) Sympathetic drive to liver and nonhepatic splanchnic tissue during heavy exercise. J Appl Physiol 82(4):1244–1249

    CAS  Google Scholar 

  • Coker RH, Simoneau L, Bülow J, Wasserman DH, Kjäer M (2001) Stimulation of splanchnic glucose production during exercise in humans contains a glucagon-independent component. Am J Physiol 280:E918–E927

    CAS  Google Scholar 

  • Collomp K, Ahmaidi S, Audran M, Chanal JL, Prefaut C (1991) Effects of caffeine ingestion on performance and anaerobic metabolism during the Wingate Test. Int J Sports Med 12(5):439–443

    CAS  PubMed  Google Scholar 

  • Delamarche A, Delamarche P (1992) Physiologie féminine et aptitude physique. Sci Motric 16:35–45

    Google Scholar 

  • Durnin JV, Rahaman MM (1967) The assessment of the amount of fat in the human body from measurements of skinfold thickness. Br J Nutr 21(3):681–689

    CAS  PubMed  Google Scholar 

  • Esbjörnsson-Liljedahl M, Sundberg CJ, Norman B, Jansson E (1999) Metabolic response in type I and type II muscle fibers during a 30-s cycle sprint in men and women. J Appl Physiol 87(4):1326–1332

    PubMed  Google Scholar 

  • Hautecouverture M, Slama G, Assan R, Tchobroutsky G (1974) Sex related diurnal variations in venous blood glucose and plasma insulin levels. Effects of estrogens in men. Diabetologia 10(6):725–730

    CAS  PubMed  Google Scholar 

  • Helge JW, Stallknecht B, Pedersen BK, Galbo H, Kiens B, Richter EA (2003) The effect of graded exercise on IL-6 release and glucose uptake in human skeletal muscle. J Physiol (Lond) 546(Pt 1):299–305

  • Howlett K, Febbraio M, Hargreaves M (1999a) Glucose production during strenuous exercise in humans: role of epinephrine. Am J Physiol 276(6 Pt 1):E1130–E1135

    CAS  PubMed  Google Scholar 

  • Howlett K, Galbo H, Lorentsen J, Bergeron R, Zimmerman-Belsing T, Bulow J, Feldt-Rasmussen U, Kjaer M (1999b) Effect of adrenaline on glucose kinetics during exercise in adrenalectomised humans. J Physiol (Lond) 519 (Pt 3):911–921

  • Jacob C, Zouhal H, Vincent S, Gratas-Delamarche A, Berthon PM, Bentue-Ferrer D, Delamarche P (2002) Training status (endurance or sprint) and catecholamine response to the Wingate test in women. Int J Sports Med 23(5):342–347

    Article  CAS  PubMed  Google Scholar 

  • Kjäer M (1998) Hepatic glucose production during exercise. Adv Exp Med Biol 441:117–127

    PubMed  Google Scholar 

  • Kjäer M, Farrell PA, Christensen NJ, Galbo H (1986) Increased epinephrine response and inaccurate glucoregulation in exercising athletes. J Appl Physiol 61(5):1693–1700

    PubMed  Google Scholar 

  • Koubi HE, Desplanche D, Gabrielle C, Cottet-Emard JM, Semporer B, Favier R (1991) Exercise endurance and fuel utilisation: a reevaluation of the effects of fasting. Appl Physiol 70:1337–1343

    Article  CAS  Google Scholar 

  • Kreisman SH, Ah Mew N, Arsenault M (2000) Epinephrine infusion during moderate-intensity exercise increases glucose production and uptake. Am J Physiol 278:E949–E957

    CAS  Google Scholar 

  • Kreisman SH, Ah Mew N, Halter JB, Vranic M, Marliss EB (2001) Norepinephrine infusion during moderate-intensity exercise increases glucose production and uptake. J Clin Endocrinol Metab 86:2118–2124

    CAS  PubMed  Google Scholar 

  • Marliss EB, Simantirakis E, Miles PD, Purdon C, Gougeon R, Field CJ, Halter JB, Vranic M (1991) Glucoregulatory and hormonal responses to repeated bouts of intense exercise in normal male subjects. J Appl Physiol 71(3):924–933

    CAS  PubMed  Google Scholar 

  • Marliss EB, Kreisman SH, Manzon A, Halter JB, Vranic M, Nessim SJ (2000) Gender differences in glucoregulatory responses to intense exercise. J Appl Physiol 88(2):457–466

    CAS  PubMed  Google Scholar 

  • Matute ML, Kalkhoff RK (1973) Sex steroid influence on hepatic gluconeogenesis and glucogen formation. Endocrinology 92(3):762–768

    CAS  PubMed  Google Scholar 

  • Moussa E, Zouhal H, Vincent S, Delamarche P, Bentué-Ferrer D, Gratas-Delamarche A (2003) Effect of sprint duration (6 s or 30 s) on plasma glucose regulation in untrained male subjects. J Sports Med Phys Fitness (in press)

  • Nevill ME, Holmyard DJ, Hall GM, Allsop P, van Oosterhout A, Burrin JM, Nevill AM (1996) Growth hormone responses to treadmill sprinting in sprint- and endurance-trained athletes. Eur J Appl Physiol 72(5–6):460–467

  • Pullinen T, Nicol C, MacDonald E, Komi PV (1999) Plasma catecholamine responses to four resistance exercise tests in men and women. Eur J Appl Physiol 80(2):125–131

    Article  CAS  Google Scholar 

  • Richter EA, Derave W, Wojtaszewski JF (2001) Glucose, exercise and insulin: emerging concepts. J Physiol (Lond) 535(Pt 2):313–322

  • Roy JY, Bongbele J, Cardin S, Brisson GR, Lavoie JM (1991) Effects of supramaximal exercise on blood glucose levels during a subsequent exercise. Eur J Appl Physiol 63(1):48–51

    CAS  Google Scholar 

  • Schnabel A, Kindermann W, Steinkraus V, Salas-Fraire O, Biro G (1984) Metabolic and hormonal responses to exhaustive supramaximal running with and without beta-adrenergic blockade. Eur J Appl Physiol 52(2):214–218

    CAS  Google Scholar 

  • Selmi A, Hautecouverture M, Basdevant A, Slama G, Tchobroutsky G (1976) Sex differences in the blood glucose fall induced by short fasts (in French). Diabete Metab 2(4):195–198

    CAS  PubMed  Google Scholar 

  • Sigal RJ, Purdon C, Bilinski D, Vranic M, Halter JB, Marliss EB (1994) Glucoregulation during and after intense exercise: effects of beta-blockade. J Clin Endocrinol Metab 78(2):359–366

    CAS  Google Scholar 

  • Sigal RJ, Fisher S, Halter JB, Vranic M, Marliss EB (1996) The roles of catecholamines in glucoregulation in intense exercise as defined by the islet cell clamp technique. Diabetes 45(2):148–156

    CAS  PubMed  Google Scholar 

  • Sigal RJ, Fisher SJ, Manzon A, Morais JA, Halter JB, Vranic M, Marliss EB (2000) Glucoregulation during and after intense exercise: effects of alpha-adrenergic blockade. Metabolism 49(3):386–394

    CAS  Google Scholar 

  • Stokes KA, Nevill ME, Hall GM, Lakomy HK (2002) Growth hormone responses to repeated maximal cycle ergometer exercise at different pedaling rates. J Appl Physiol 92(2):602–608

    CAS  Google Scholar 

  • Tarnopolsky LJ, MacDougall JD, Atkinson SA, Tarnopolsky MA, Sutton JR (1990) Gender differences in substrate for endurance exercise. J Appl Physiol 68(1):302–308

    CAS  PubMed  Google Scholar 

  • Tarnopolsky MA, Atkinson SA, Phillips SM, MacDougall JD (1995) Carbohydrate loading and metabolism during exercise in men and women. J Appl Physiol 78(4):1360–1368

    CAS  PubMed  Google Scholar 

  • Tarnopolsky MA, Bosman M, Macdonald JR, Vandeputte D, Martin J, Roy BD (1997) Postexercise protein-carbohydrate and carbohydrate supplements increase muscle glycogen in men and women. J Appl Physiol 83(6):1877–1883

    CAS  PubMed  Google Scholar 

  • Tchobroutsky G (1991) Blood glucose levels in diabetic and non-diabetic subjects. Diabetologia 34(2):67–73

    CAS  PubMed  Google Scholar 

  • Van Beaumont W, Strand JC, Petrofsky JS, Hipskind SG, Greenleaf JE (1973) Changes in total plasma content of electrolytes and proteins with maximal exercise. J Appl Physiol 34(1):102–106

    Google Scholar 

  • Vandewalle H, Peres G, Heller J, Monod H (1985) All out anaerobic capacity tests on cycle ergometers. A comparative study on men and women. Eur J Appl Physiol 54(2):222–229

    Google Scholar 

  • Zouhal H, Gratas-Delamarche A, Bentue-Ferrer D, Rannou F, Granier P, Delamarche P (1998) Réponses des catécholamines plasmatiques à l’exercice supramaximal chez des endurants. Sci Sports 13:112–118

    Article  Google Scholar 

Download references

Acknowledgements

The authors thank nurses Dominique Paul and Marie-Thérèse Gougeon, and laboratory technician Yolande Briand for technical assistance, thank Kathy Stephen and Michelle Jester for English language correction and thank all the volunteers for their participation in this study. The experiments used in this article complied with the current laws of France.

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Correspondence to Sophie Vincent.

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Vincent, S., Berthon, P., Zouhal, H. et al. Plasma glucose, insulin and catecholamine responses to a Wingate test in physically active women and men. Eur J Appl Physiol 91, 15–21 (2004). https://doi.org/10.1007/s00421-003-0957-5

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  • DOI: https://doi.org/10.1007/s00421-003-0957-5

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