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Influence of glucose and insulin on transcapillary fluid absorption from the arm during lower body negative pressure in man

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Abstract

This study examined the influence of insulin and glucose on the transcapillary fluid absorption during lower body negative pressure (LBNP) in humans. Ten healthy males [23 (1) years] were exposed to LBNP of 45 cmH2O on two occasions: (1) before and during a hyperinsulinaemic clamp (HI) and (2) before and during a hyperglycaemic clamp (HG). Transcapillary fluid absorption and blood flow were recorded with volumetric technique. Forearm blood flow increased during HI from 2.3 (0.3) ml (100 ml)−1 min−1 to 3.3 (0.5) ml (100 ml)−1 min−1 (P<0.05). The haemodynamic response to LBNP was similar during HI and HG compared with control LBNP. Transcapillary fluid absorption during LBNP increased during HG from 0.044 (0.007) ml (100 ml)−1 min−1 to 0.059 (0.009) ml (100 ml)−1 min−1 (P<0.01), whereas it was unchanged during HI. In conclusion, hyperglycaemia augments transcapillary fluid absorption from skeletal muscle and skin during LBNP whereas hyperinsulinaemia has no such effect. This indicates that in human hyperglycaemia contributes to plasma volume restitution during hypovolaemic circulatory stress.

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Acknowledgements

Grants from Novo Foundation, Denmark, the Medical Faculty, Lund University, the Medical Research Council (no. 12661), the Swedish Heart Lung Foundation and the Funds of Malmö University Hospital supported this study.

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Authors and Affiliations

  1. Department of Internal Medicine, Helsingborg Hospital, 251 87, Helsingborg, Sweden

    H. Olsen

  2. Department of Endocrinology, Malmö University Hospital, Malmö, Sweden

    L. Groop

  3. Department of Medicine and Care, Linköping University, Linköping, Sweden

    T. Länne

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  1. H. Olsen
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  2. L. Groop
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  3. T. Länne
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Correspondence to H. Olsen.

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Olsen, H., Groop, L. & Länne, T. Influence of glucose and insulin on transcapillary fluid absorption from the arm during lower body negative pressure in man. Eur J Appl Physiol 90, 138–143 (2003). https://doi.org/10.1007/s00421-003-0894-3

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