Abstract
Orai1 is an essential pore-forming subunit of the Ca2+ release-activated Ca2+ channels and plays a key role in the store-operated Ca2+ entry. However, little is known about the function of this pathway in allergic airway diseases. In this study, we evaluated Orai1 expression in normal and allergic rhinitis (AR) mice airway and spleen. AR models were established by repetitive intraperitoneal sensitization followed by intranasal challenge with ovalbumin. Sneezing was counted, and eosinophils infiltration was analyzed through Luna stain. We performed the analysis of Orai1 protein in airway and spleen by immunohistochemical staining, Western blotting and enzyme-linked immunosorbent assay, and quantitatively examined Orai1 mRNA in the above tissues by real-time reverse transcription-polymerase chain reaction. Sneezes and eosinophil counts in the AR group were increased in comparison to those in the normal group. Orai1 protein was expressed in mucosal epithelium and submucosal glands epithelium of airway, and in immune cells of spleen. The immunostaining appeared stronger in AR mice than that in normal ones. Both the Orai1 protein and mRNA levels showed up-regulation in the AR group compared with those in the normal one. Our results indicate that Orai1 is up-regulated in the airway and spleen in allergic inflammation and may participate in the pathogenesis of allergic rhinitis.
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Abbreviations
- AR:
-
Allergic rhinitis
- Th2:
-
T helper 2
- IL:
-
Interleukin
- CRAC:
-
Ca2+ release-activated Ca2+
- STIM1:
-
Stromal interaction molecule 1
- SOCE:
-
Store-operated Ca2+ entry
- NFAT:
-
Nuclear factor of activated T cells
- RT-PCR:
-
Reverse transcription-polymerase chain reaction
- ELISA:
-
Enzyme-linked immunosorbent assay
- NIH:
-
National Institutes of Health
- i.p.:
-
Intraperitoneal
- i.n.:
-
Intranasal
- PBS:
-
Phosphate-buffered saline
- DAB:
-
3,3′-Diaminobenzidine chromogene
- TBS:
-
Tris-buffered saline
- TBS-T:
-
Tris-buffered saline with Tween-20
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Lin, L., Zheng, C., Zhang, L. et al. Up-regulation of Orai1 in murine allergic rhinitis. Histochem Cell Biol 134, 93–102 (2010). https://doi.org/10.1007/s00418-010-0717-7
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DOI: https://doi.org/10.1007/s00418-010-0717-7