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Heterogeneous distribution of TRPC proteins in the embryonic cortex

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Abstract

The present study was initiated to gain some information about the tissue distribution of transient receptor potential proteins of C-type (TRPC), a family of voltage-independent cation channels, at the beginning of neurogenesis in the telencephalon of embryonic mice. The mRNAs of all known TRPCs (TRPC1–TRPC7) could be found in the cortex at E13. TRPC1, TRPC3 and TRPC5 were the main isoforms, whereas the mRNAs for TRPC2, TRPC4, TRPC6 and TRPC7 were less abundant. The distribution throughout the cortical wall of TRPC1, TRPC3 and TRPC6 was studied by means of immuno-histochemistry. The data collected pointed to a heterogeneous expression of the channels. Three groups were identified. The first one comprises TRPC1, specifically found in the preplate but only in some post-mitotic neurons. It was mainly observed in a subset of cells distinct from the Cajal-Retzius cells. The second group is composed of TRPC3. It was found in non-neuronal cells and also in dividing (5-bromo-2′-deoxyuridine-positive) cells, indicating that TRPC3 is present in precursor cells. The third group contains TRPC6 detected in neuronal and in dividing non-neuronal cells. Double immunostaining experiments showed that TRPC3-positive cells also express TRPC6. Collectively, this report highlights a specific TRPC expression pattern in the immature cortical wall.

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Acknowledgments

We wish to thank D. Grunwald for his help with the Leica scanning confocal microscope (iRTSV, CEA, Grenoble) and L. Lemonnier for helpful comments. This study was supported by a grant from 1’Agence Nationale de la Recherche (ANR).

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Authors and Affiliations

  1. Institut des Neurosciences, Inserm U837, Grenoble, France

    Sylvie Boisseau

  2. EMAU Greifswald, Institut für Pathophysiologie, 17495, Karlsburg, Germany

    Christiane Kunert-Keil & Silke Lucke

  3. Laboratoire de Chimie et Biologie des Métaux, UMR CNRS 5249, CEA, 17 rue des Martyrs, 38054, Grenoble, France

    Alexandre Bouron

  4. CEA, DSV, iRTSV, Grenoble, France

    Alexandre Bouron

  5. Université Joseph Fourier, Grenoble, France

    Sylvie Boisseau & Alexandre Bouron

Authors
  1. Sylvie Boisseau
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  2. Christiane Kunert-Keil
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  3. Silke Lucke
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  4. Alexandre Bouron
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Correspondence to Alexandre Bouron.

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S. Boisseau and C. Kunert-Keil have contributed equally to this work.

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Boisseau, S., Kunert-Keil, C., Lucke, S. et al. Heterogeneous distribution of TRPC proteins in the embryonic cortex. Histochem Cell Biol 131, 355–363 (2009). https://doi.org/10.1007/s00418-008-0532-6

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