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Nuclear presence of adhesion-/growth-regulatory galectins in normal/malignant cells of squamous epithelial origin

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Abstract

Cellular activities in the regulation of growth or adhesion/migration involve protein (lectin)–carbohydrate recognition at the cell surface. Members of the galectin family of endogenous lectins additionally bind distinct intracellular ligands. These interactions with protein targets explain the relevance of their nuclear and cytoplasmic presence. Expression profiling for galectins and accessible binding sites is a histochemical approach to link localization with cellular growth properties. Non-cross-reactive antibodies for the homodimeric (proto-type) galectins-1, -2 and -7 and the chimera-type galectin-3 (Gal-3) as well as the biotinylated lectins were tested. This analysis was performed with the FaDu squamous carcinoma cell line and long-term cultured human and porcine epidermal cells as models for malignant and normal cells of squamous cell epithelial origin. A set of antibodies was added for phenotypic cell characterization. Strong nuclear and cytoplasmic signals of galectins and the differential reactivity of labeled galectins support the notion of their individual properties. The length of the period of culture was effective in modulating marker expression. Cytochemical expression profiling is a prerequisite for the selection of distinct proteins for targeted modulation of gene expression as a step toward functional analysis.

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Acknowledgements

This study was supported by the Grant Agency of the Czech Republic, project no. 304/04/0171, the Ministry of Education, Youth and Sport, project nos. MSM0021620806 and 1M0021620803 and the Mizutani Foundation for Glycoscience. We thank Dr. P. Hozák, Dr. h. c. mult. K. Kayser and Dr. S. Namirha for helpful comments and Dr. F.-T. Liu for kindly providing Gal-3-specific monoclonal antibody A1D6. The authors are grateful to E. Vancová for excellent technical assistance.

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Authors and Affiliations

  1. Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic

    Karel Smetana Jr & Barbora Dvořánková

  2. Center of Cell Therapy and Tissue Repair, 2nd Faculty of Medicine, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic

    Karel Smetana Jr, Barbora Dvořánková, Jiří Klíma & Jan Motlík

  3. Department of Otorhinolaryngology, Head and Neck Surgery, Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic

    Martin Chovanec & Jan Bouček

  4. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 140 08, Prague 4, Czech Republic

    Jan Bouček

  5. Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburská 89, 277 21, Liběchov, Czech Republic

    Jiří Klíma & Jan Motlík

  6. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstrasse 13, 80539, Munich, Germany

    Martin Lensch, Herbert Kaltner, Sabine André & Hans Joachim Gabius

Authors
  1. Karel Smetana Jr
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  2. Barbora Dvořánková
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  3. Martin Chovanec
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  4. Jan Bouček
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  5. Jiří Klíma
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  6. Jan Motlík
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  7. Martin Lensch
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  8. Herbert Kaltner
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  9. Sabine André
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  10. Hans Joachim Gabius
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Corresponding author

Correspondence to Karel Smetana Jr.

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Smetana, K., Dvořánková, B., Chovanec, M. et al. Nuclear presence of adhesion-/growth-regulatory galectins in normal/malignant cells of squamous epithelial origin. Histochem Cell Biol 125, 171–182 (2006). https://doi.org/10.1007/s00418-005-0074-0

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  • DOI: https://doi.org/10.1007/s00418-005-0074-0

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