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Carboxypeptidase E in rat antropyloric mucosa: distribution in progenitor and mature endocrine cell types

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Abstract

Processing of most gut hormones involves cleavage between dibasic amino acids followed by carboxypeptidase-catalyzed removal of the COOH-terminal basic residue, resulting in peptides with a COOH-terminal glycine. Such peptides may subsequently be converted to amidated peptides or can be directly secreted. It is believed that carboxypeptidase E (CPE) is involved in gut hormone processing but its presence in gut endocrine cells has never been studied. We have analyzed the distribution of CPE in the antropyloric mucosa of rat stomach and report that gastrin cells and progenitor gastrin-somatostatin (G/D) cells express CPE while mature somatostatin cells and the majority of serotonin cells fail to express CPE. These data indicate that immature G/D cells are able to process gastrin to glycine-extended forms and that CPE-mediated processing is not a characteristic of mature somatostatin and serotonin cells.

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Acknowledgements

Grant support was from the Danish Agricultural and Veterinary Research Council, the Danish Medical Research Council and the Danish Cancer Society.

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Authors and Affiliations

  1. Division of Cell Biology, Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Gronnegaardsvej 7, 1870 Frederiksberg C, Copenhagen, Denmark

    Lars-Inge Larsson

  2. Department of Clinical Biochemistry, Statens Serum Institut, Copenhagen, Denmark

    David M. Hougaard

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  1. David M. Hougaard
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  2. Lars-Inge Larsson
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Correspondence to Lars-Inge Larsson.

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Hougaard, D.M., Larsson, LI. Carboxypeptidase E in rat antropyloric mucosa: distribution in progenitor and mature endocrine cell types. Histochem Cell Biol 121, 55–61 (2004). https://doi.org/10.1007/s00418-003-0606-4

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