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The association between systemic oxidative stress and ocular blood flow in patients with normal-tension glaucoma

  • Glaucoma
  • Published:
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Abstract

Purpose

To evaluate the association between ocular blood flow and biomarkers of systemic oxidative stress, as well as the potential of these biomarkers to assess normal-tension glaucoma (NTG).

Methods

This study included 73 eyes of 73 patients with NTG. We assessed ocular blood flow by measuring mean blur rate (MBR) in the optic nerve head using laser speckle flowgraphy, both overall and separately in the vessel and tissue areas. We also measured urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG) and skin autofluorescence (SAF), and lastly, determined correlations between these measurements and with other clinical parameters.

Results

SAF was correlated with age, circumpapillary retinal nerve fiber layer thickness (cpRNFLT), mean deviation (MD), and overall MBR (P = 0.003, P = 0.013, P = 0.015 and P = 0.006, respectively). SAF and 8-OHdG were both correlated with tissue-area MBR (P = 0.006 and P = 0.010, respectively). Visual acuity, cpRNFLT, mean deviation and tissue-area MBR had a significant tendency to change with NTG severity (P = 0.014, P < 0.001, P < 0.001 and P = 0.006, respectively). Multiple regression analysis revealed that cpRNFLT and 8-OHdG were independent contributing factors to MD (P < 0.001 and P = 0.040, respectively), and that cpRNFLT and 8-OHdG were independent contributing factors to tissue-area MBR (P = 0.005 and P = 0.028, respectively).

Conclusions

We found a close relationship between cpRNFLT, MD, tissue MBR, SAF and 8-OHdG, suggesting that systemic oxidative stress is associated with decreased ocular blood flow and may be involved in the pathogenesis of NTG.

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Acknowledgments

We thank Mr. Tim Hilts for reviewing and editing the language of the manuscript, and Ms. Akane Enta and Mr. Nobuo Akiyama for technical assistance. This paper was supported in part by a JSPS KAKEN Grant-in-Aid for young scientists (N.H, 26861434), a JST grant from JSPS KAKENHI Grants-in-Aid for Scientific Research (B) (T.N. 26293372) and for Exploratory Research (T.N. 26670751), and by the JST Center for Revitalization Promotion. There is no commercial financial support to report. There were no competing financial interests in this study.

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Authors and Affiliations

  1. Department of Ophthalmology, Tohoku University Graduate School of Medicine, 1-1 Seiryo, Aoba, Sendai, Miyagi, 980-8574, Japan

    Noriko Himori, Hiroshi Kunikata, Yukihiro Shiga, Kazuko Omodaka, Kazuichi Maruyama, Hidetoshi Takahashi & Toru Nakazawa

  2. Department of Retinal Disease Control, Tohoku University Graduate School of Medicine, Sendai, Japan

    Hiroshi Kunikata & Toru Nakazawa

  3. Department of Advanced Ophthalmic Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan

    Toru Nakazawa

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  1. Noriko Himori
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  2. Hiroshi Kunikata
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  3. Yukihiro Shiga
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  4. Kazuko Omodaka
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  5. Kazuichi Maruyama
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Correspondence to Toru Nakazawa.

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All authors certify that they have no affiliations with or involvement in any organization or entry with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Himori, N., Kunikata, H., Shiga, Y. et al. The association between systemic oxidative stress and ocular blood flow in patients with normal-tension glaucoma. Graefes Arch Clin Exp Ophthalmol 254, 333–341 (2016). https://doi.org/10.1007/s00417-015-3203-z

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  • DOI: https://doi.org/10.1007/s00417-015-3203-z

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