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Plasma and whole-blood chemokine levels in patients with Behcet's disease

  • Clinical Investigation
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Background

Chemokines are a family of chemoattractants of leukocytes that play a critical role for leukocyte recruitment in various inflammatory diseases. The purpose of this study is to investigate the involvement of chemokines, interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in the peripheral blood, with a special reference to disease activities of the patients with Behçet's disease (BD).

Methods

The study population consisted of totally 55 patients with BD who had panuveitis (20 patients with active BD, 35 patients with inactive BD) as well as 19 healthy volunteers as control. Disease activity was defined according to the existence of ocular inflammation. IL-8 and MCP-1 concentration levels in the plasma and whole-blood samples were measured by enzyme-linked immunosorbent assay. Whole-blood samples were obtained by lysing cell membranes of peripheral blood cells.

Results

Most of the plasma IL-8 samples were below the detectable limit. Whole-blood IL-8 levels were readily measured. The levels in the patients with active BD were significantly higher than the other two groups. The patients with active and inactive BD showed higher plasma and whole-blood levels of MCP-1 than controls. The plasma and whole-blood MCP-1 levels of the samples collected at the same time showed a linear correlation.

Conclusion

A close relationship was found to exist between the cell-associated IL-8 and the disease activity, while a persistent role of MCP-1 was observed in BD. Measuring the whole-blood levels of chemokines is useful for monitoring the disease activity.

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Acknowledgements

This work was in part supported by a grant for research on Behcet's disease from the Ministry of Health, Welfare, and Labor of Japan.

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Authors and Affiliations

  1. Department of Ophthalmology, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, 113-8655, Tokyo , Japan

    Toshikatsu Kaburaki, Hidetoshi Kawashima, Jiro Numaga & Jun Chen

  2. Department of Ophthalmology, The Tokyo Kosei-Nenkin Hospital, Tokyo, Japan

    Yujiro Fujino

  3. Department of Ophthalmology, School of Medicine, Catholic University of Santiago, Santiago, Chile

    Guillermo Merino

  4. Department of Molecular Preventive Medicine, University of Tokyo Graduate School of Medicine, Tokyo, Japan

    Kouji Matsushima

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  1. Toshikatsu Kaburaki
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  2. Yujiro Fujino
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  3. Hidetoshi Kawashima
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  4. Guillermo Merino
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  5. Jiro Numaga
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  6. Jun Chen
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  7. Kouji Matsushima
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Correspondence to Toshikatsu Kaburaki.

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Kaburaki, T., Fujino, Y., Kawashima, H. et al. Plasma and whole-blood chemokine levels in patients with Behcet's disease. Graefe's Arch Clin Exp Ophthalmol 241, 353–358 (2003). https://doi.org/10.1007/s00417-003-0668-y

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  • DOI: https://doi.org/10.1007/s00417-003-0668-y

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