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Developmental validation of the HomyGene19+14Y System

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Abstract

The HomyGene19+14Y System (HG19+14Y) is a PCR-based amplification kit that enables typing of 18 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, Penta E, TPOX, TH01, vWA), 14 widely used Y chromosome STR (Y-STR) loci (Y_GATA_H4, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS456, DYS458, DYS635), and amelogenin. This multiplex system was designed for the simultaneous analysis of amelogenin-Y allele mutation, single-source searches, kinship (including familial searching), mixture profiles, international data sharing, and other forensic applications. In this study, the multiplex system was validated for sensitivity, specificity, DNA mixtures, stability, precision, stutter, reproducibility, parallel tests, PCR-based conditions, and population analysis according to the Scientific Working Group on DNA Analysis Methods (SWGDAM) developmental validation guidelines. A total of 212 alleles were detected for the 18 autosomal STR loci among 528 Guangdong Han individuals, and 431 haplotypes were found for 14 Y-STRs among 452 unrelated males. The combined match probability (CMP) of the HG19+14Y System was calculated as 2.39 × 10−29. All the validation results showed that the HG19+14Y System would be a robust, reliable, highly polymorphic, and informative forensic kit.

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Acknowledgements

This work was supported by a grant from Science and Technology Planning Project of Guangdong Province, China (Grant No. 2013B021500010), Natural Science Foundation of Guangdong Province (Grant No. 2014A030310025), and Medical Science and Technology Research Foundation of Guangdong Province (A2015043).

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Authors and Affiliations

  1. School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China

    Weian Du, Ling Chen, Pingming Qiu & Chao Liu

  2. Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China

    Hong Liu & Chao Liu

  3. AGCU ScienTech Incorporation, Wuxi, 214174, China

    Fayuan Li

  4. HOMY GeneTech Incorporation, Foshan, 518000, China

    Jing Gao, Yu Zhou & Bangchao Wang

Authors
  1. Weian Du
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  2. Ling Chen
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  3. Hong Liu
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  4. Pingming Qiu
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  5. Fayuan Li
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  7. Yu Zhou
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  8. Bangchao Wang
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  9. Chao Liu
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Correspondence to Chao Liu.

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The authors declare that they have no conflict of interest.

Additional information

Weian Du and Ling Chen contributed equally to this work

Electronic supplementary material

Table S1

The allele frequencies and forensic parameters of 18 autosomal STR loci in Guangdong Han population (n = 528) (DOCX 35 kb)

Table S2

The allele frequencies and GD values of 14 Y-STR loci in Guangdong Han male population (n = 452) (DOCX 24 kb)

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Du, W., Chen, L., Liu, H. et al. Developmental validation of the HomyGene19+14Y System. Int J Legal Med 131, 605–620 (2017). https://doi.org/10.1007/s00414-016-1505-2

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