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Two X family DNA polymerases, λ and μ, in meiotic tissues of the basidiomycete, Coprinus cinereus

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Abstract

The X family DNA polymerases λ (CcPolλ) and μ (CcPolμ) were shown to be expressed during meiotic prophase in the basidiomycete, Coprinus cinereus. These two polymerases are the only members of the X family in the C. cinereus genome. The open reading frame of CcPolλ encoded a predicted product of 800 amino acid residues and that of CcPolμ of 621 amino acid residues. Both CcPolλ and CcPolμ required Mn2+ ions for activity, and both were strongly inhibited by dideoxythymidine triphosphate. Unlike their mammalian counterparts, CcPolλ and CcPolμ had no terminal deoxynucleotidyl transferase activity. Immunostaining analysis revealed that CcPolλ was present at meiotic prophase nuclei in zygotene and pachytene cells, which is the period when homologous chromosomes pair and recombine. CcPolμ was present in a slightly wider range of cell stages, zygotene to diplotene. In analyses using D-loop recombination intermediate substrates, we found that both CcPolλ and CcPolμ could promote primer extension of an invading strand in a D-loop structure. Moreover, both polymerases could fully extend the primer in the D-loop substrate, suggesting that D-loop extension is an activity intrinsic to CcPolλ and CcPolμ. Based on these data, we discuss the possible roles of these polymerases in meiosis.

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Acknowledgment

We thank Dr. Nara T, Dr. Hamada F, and Dr. Namekawa S for technical assistance and helpful discussions

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Authors and Affiliations

  1. Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken, 278-8510, Japan

    Aiko Sakamoto, Kazuki Iwabata, Akiyo Koshiyama, Hiroko Sugawara, Takuro Yanai, Yoshihiro Kanai, Ryo Takeuchi, Yoko Daikuhara, Yoichi Takakusagi & Kengo Sakaguchi

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  1. Aiko Sakamoto
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  2. Kazuki Iwabata
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  3. Akiyo Koshiyama
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  10. Kengo Sakaguchi
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Correspondence to Kengo Sakaguchi.

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Communicated by P. Shaw

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Sakamoto, A., Iwabata, K., Koshiyama, A. et al. Two X family DNA polymerases, λ and μ, in meiotic tissues of the basidiomycete, Coprinus cinereus . Chromosoma 116, 545–556 (2007). https://doi.org/10.1007/s00412-007-0119-3

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