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Recruitment of IL-27-Producing CD4+ T Cells and Effect of IL-27 on Pleural Mesothelial Cells in Tuberculous Pleurisy

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Abstract

Background

The numbers of IL-27-producing CD4+ T cells and the concentration of soluble IL-27 have been found to be increased in tuberculous pleural effusion (TPE). The objective of the present study was to explore the mechanism by which IL-27+CD4+ T cells are recruited into the pleural space, and to explore the impact of IL-27 on pleural mesothelial cells (PMCs).

Methods

The expression profiles of chemokine receptor (CCR) were determined by flow cytometry. The chemoattractant activity of chemokines CCL20 and CCL22 for IL–27+CD4+ T cells in vitro was observed. Effects of IL-27 on wound healing, proliferation and apoptosis of PMCs were also investigated.

Results

IL-27+CD4+ T cells in TPE expressed high level of CCR6, medium level of CCR4, and low levels of CCR2, CCR3, CCR5, CCR7, CCR10, and CXCR3. Recruitment of IL-27+CD4+ T cells into TPE could be induced by pleural CCL20 and CCL22. By activating STAT3 signaling, IL-27 significantly improved wound healing and promoted proliferation of PMCs, and completely prevented apoptosis of PMCs induced by IFN-γ.

Conclusions

After being recruited into pleural space by CCL20 or/and CCL22, these pleural IL-27-producing CD4+ T cells may play important roles in tuberculosis immunity by affecting PMC functions.

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Acknowledgments

This work was supported in part by grants from the National Natural Science Foundation of China (Nos. 81270149, 91442109, 31470883, and 81400069), in part, and a grant from the National Basic Research Program of China 973 Program (No. 2012CB518706), and in part by the High-Level Technical Personnel Training Project of Beijing Municipal Health System, China (No. 2013-2-010).

Conflicts of interest

The authors declare that there are no conflicts of interest.

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    Authors and Affiliations

    1. Department of Respiratory Medicine, First People’s Hospital of Foshan, Sun Yat-sen University, Foshan, China

      Zhi-Jian Ye

    2. Department of Respiratory and Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, China

      Zhi-Jian Ye, Li-Li Xu, Ai Cui, Xiao-Juan Wang, Zhen Wang, Zhao-Hui Tong & Huan-Zhong Shi

    3. Center of Medical Research, Beijing Institute of Respiratory Diseases, Beijing, China

      Kan Zhai & Huan-Zhong Shi

    4. Department of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

      Qiong Zhou

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    1. Zhi-Jian Ye
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    Corresponding author

    Correspondence to Huan-Zhong Shi.

    Additional information

    Zhi-Jian Ye and Li-Li Xu have contributed equally to the present work.

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    Ye, ZJ., Xu, LL., Zhou, Q. et al. Recruitment of IL-27-Producing CD4+ T Cells and Effect of IL-27 on Pleural Mesothelial Cells in Tuberculous Pleurisy. Lung 193, 539–548 (2015). https://doi.org/10.1007/s00408-015-9738-2

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    • DOI: https://doi.org/10.1007/s00408-015-9738-2

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