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Prevalence and significance of human parvovirus variants in skin from primary cutaneous T cell lymphomas, inflammatory dermatoses and healthy subjects

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Abstract

Primary cutaneous T cell lymphomas (CTCL) represent a heterogeneous group of T lymphomas. Virus involvement in CTCL pathogenesis has been extensively investigated, but no data are available as to a causative role of parvovirus B19. The prevalence of parvovirus variants (B19, LaL1/K71, V9) was investigated by using two nested PCRs and a genotype-2 semiquantitative PCR (Q-PCR). Parvovirus DNA was detected in similar percentage in healthy skin controls (40%; n = 42), inflammatory dermatoses (ID) (41%; n = 80) and CTCL (34%; n = 76). Among variants, genotype-2 was more prevalent in ID (26%) and CTCL (22%) than in normal skin (14%; p < 0.05). genotype-3 was never found in normal skin and was rare in ID. The only four pathological skin samples with a quantifiable genome copies/μg DNA values in Q-PCR were ID. In conclusion, despite the skin represent a reservoir for genotype-1, parvovirus infection is not involved in the etiopathogenesis of CTCL.

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References

  1. Baskan EB, Yilmaz E, Saricaoglu H et al (2007) Detection of parvovirus B19 DNA in the lesional skin of patients with Behçet’s disease. Clin Exp Dermatol 32:186–190. doi:10.1111/j.1365-2230.2006.02330.x

    Article  PubMed  CAS  Google Scholar 

  2. Bergallo M, Costa C, Sidoti F et al (2008) Variants of parvovirus B19: bioinformatical evaluation of nested PCR assays and genotypes detection. Intervirol 51:75–80. doi:10.1159/000127429

    Article  CAS  Google Scholar 

  3. Bernengo MG, Novelli M, Quaglino P et al (2001) The relevance of the CD4 + CD26—subset in the identification of circulating Sezary cells. Br J Dermatol 144:124–135. doi:10.1046/j.1365-2133.2001.04014.x

    Article  Google Scholar 

  4. Brown KE, Young NS (1997) Parvovirus B19 in human disease. Annu Rev Med 48:59–67. doi:10.1146/annurev.med.48.1.59

    Article  PubMed  CAS  Google Scholar 

  5. Chevrel G, Calyet A, Belin V et al (2000) Dermatomyositis associated with the presence of parvovirus B19 DNA in muscle. Rheumatology (Oxford) 39:1037–1039. doi:10.1093/rheumatology/39.9.1037

    Article  CAS  Google Scholar 

  6. Crowson AN, Magro CN, Dawood MR (2000) A causal role for parvovirus B19 infection in adult dermatomyositis and other autoimmune syndromes. J Cutan Pathol 27:505–515. doi:10.1034/j.1600-0560.2000.027010505.x

    Article  PubMed  CAS  Google Scholar 

  7. Drago F, Semino M, Rampini P et al (1999) Parvovirus B19 infection associated with acute hepatitis and a purpuric exanthem. Br J Dermatol 141:160–161. doi:10.1046/j.1365-2133.1999.02943.x

    Article  PubMed  CAS  Google Scholar 

  8. Ekman A, Hokynar K, Kakkola L et al (2007) Biological and immunological relations among Human Parvovirus B19 genotypes 1 to 3. J Virol 81:6927–6935. doi:10.1128/JVI.02713-06

    Article  PubMed  CAS  Google Scholar 

  9. Ferri C, Azzi A, Magro CM (2005) Parvovirus B19 and systemic sclerosis. Br J Dermatol 152:819–820. doi:10.1111/j.1365-2133.2005.06515.x

    Article  PubMed  CAS  Google Scholar 

  10. Florea AV, Ionescu DN, Melhem MF (2007) Parvovirus B19 infection in the immunocompromised host. Arch Pathol Lab Med 131:799–804

    PubMed  Google Scholar 

  11. Heegaard ED, Panum Jensen I, Christensen J (2001) Novel PCR assay for differential detection and screening of erythrovirus B19 and erythrovirus V9. J Med Virol 65:362–367. doi:10.1002/jmv.2042

    Article  PubMed  CAS  Google Scholar 

  12. Heegaard ED, Brown KE (2002) Human parvovirus B19. Clin Microbiol Rev 15:485–505. doi:10.1128/CMR.15.3.485-505.2002

    Article  PubMed  Google Scholar 

  13. Hokynar K, Soderlund-Venermo M, Pesonen M et al (2002) A new parvovirus genotype-persistent in human skin. Virology 302:224–228. doi:10.1006/viro.2002.1673

    Article  PubMed  CAS  Google Scholar 

  14. Katta R (2002) Parvovirus B19: a review. Dermatol Clin 20:333–342. doi:10.1016/S0733-8635(01)00013-4

    Article  PubMed  Google Scholar 

  15. Kerr S, O’keeffe G, Kilty C et al (1999) Undenatured parvovirus B19 antigens are essential for the accurate detection of parvovirus B19 IgG. J Med Virol 57:179–185. doi:10.1002/(SICI)1096-9071(199902)57:2<179::AID-JMV16>3.0.CO;2-T

    Article  PubMed  CAS  Google Scholar 

  16. Labarthe MP, Salomon D, Saurat JH (1996) Ulcération linguales, parapsoriasis en gouttes et primo-infection a parvovirus B19. Ann Dermatol Venereol 123:735–738

    PubMed  CAS  Google Scholar 

  17. Magro CM, Dawood MR, Crowson AN (2000) The cutaneous manifestations of human parvovirus B19 infection. Hum Pathol 31:488–497. doi:10.1053/hp.2000.6714

    Article  PubMed  CAS  Google Scholar 

  18. Nagore E, Ledesda E, Collado C et al (2000) Detection of Epstein–Barr virus and human herpesvirus 7 and 8 genomes in primary cutaneous T and B-cell lymphomas. Br J Dermatol 14:320–323. doi:10.1046/j.1365-2133.2000.03657.x

    Article  Google Scholar 

  19. Nguyen QT, Sifer C, Schneider V et al (1999) Novel human erythrovirus associated with transient aplastic anemia. J Clin Microbiol 37:2483–2487

    PubMed  CAS  Google Scholar 

  20. Nguyen QT, Wong S, Heegaard ED et al (2002) Identification and characterization of a second novel human erythrovirus variant, A6. Virology 301:374–380. doi:10.1006/viro.2002.1585

    Article  PubMed  CAS  Google Scholar 

  21. Norja P, Hokynar K, Aaltonen LM et al (2006) Bioportfolio: lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue. Proc Natl Acad Sci USA 103:7450–7474. doi:10.1073/pnas.0602259103

    Article  PubMed  CAS  Google Scholar 

  22. Novelli M, Merlino C, Ponti R et al. (2009) Epstein-Barr Virus in cutaneous T-Cell Lymphomas: evaluation of the viral presence and significance in skin and peripheral blood. J Invest Dermatol. doi:10.1038/jid.2008.396

  23. Ofuji S, Yamamoto O (2007) Acute generalized exanthematous pustulosis associated with a human parvovirus B19 infection. J Dermatol 34:121–123. doi:10.1111/j.1346-8138.2006.00230.x

    Article  PubMed  Google Scholar 

  24. Olsen E, Vonderheid E, Pimpinelli N et al (2007) Revisions to the staging and classification of mycosis fungoides and Sezary syndrome: a proposal of the International Society for Cutaneous Lymphomas (ISCL) and the cutaneous lymphoma task force of the European Organization of Research and Treatment of Cancer (EORTC). Blood 110:1713–1722. doi:10.1182/blood-2007-03-055749

    Article  PubMed  CAS  Google Scholar 

  25. Ohtsuka T, Yamazaki S (2005) Altered prevalence of human parvovirus B19 component genes in systemic sclerosis skin tissue. Br J Dermatol 152:1078–1080. doi:10.1111/j.1365-2133.2005.06567.x

    Article  PubMed  CAS  Google Scholar 

  26. Parsyan A, Kerr S, Owusu-Ofori S (2006) Reactivity of genotype-specific recombinant proteins of human erythrovirus B19 with plasmas from areas where genotype 1 or 3 is endemic. J Clin Microbiol 44:1367–1375. doi:10.1128/JCM.44.4.1367-1375.2006

    Article  PubMed  CAS  Google Scholar 

  27. Ponti R, Quaglino P, Novelli M et al (2005) T-cell receptor gamma gene rearrangement by multiplex polymerase chain reaction/heteroduplex analysis in patients with cutaneous T-cell lymphoma (mycosis fungoides/Sézary syndrome) and benign inflammatory disease: correlation with clinical, histological and immunophenotypical findings. Br J Dermatol 153:565–573. doi:10.1111/j.1365-2133.2005.06649.x

    Article  PubMed  CAS  Google Scholar 

  28. Ponti R, Fierro MT, Quaglino P et al (2008) TCRgamma-chain gene rearrangement by PCR-based GeneScan: diagnostic accuracy improvement and clonal heterogeneity analysis in multiple cutaneous T-cell lymphoma samples. J Invest Dermatol 128:1030–1038. doi:10.1038/sj.jid.5701109

    Article  PubMed  CAS  Google Scholar 

  29. Ponti R, Bergallo M, Costa C et al (2008) Human Herpesvirus 7 detection by real time quantitative PCR in primary cutaneous T cell lymphomas and healthy subjects: lack of a pathogenic role. Br J Dermatol 159:1131–1137. doi:10.1111/j.1365-2133.2008.08575.x

    Article  PubMed  CAS  Google Scholar 

  30. Reid DM, Reid TMS, Brown T et al (1985) Human parvovirus associated arthritis: a clinical and laboratory description. Lancet 1:422–425. doi:10.1016/S0140-6736(85)91146-8

    Article  PubMed  CAS  Google Scholar 

  31. Schwarz TF, Wiersbitzky S, Pambor M (1994) Case report: detection of parvovirus B19 in a skin biopsy of a patient with erythema infectiosum. J Med Virol 43(2):171–174. doi:10.1002/jmv.1890430214

    Article  PubMed  CAS  Google Scholar 

  32. Servant A, Laperche S, Lallemand F et al (2002) Genetic diversity within human erythroviruses: identification of three genotypes. J Virol 76:9124–9134. doi:10.1128/JVI.76.18.9124-9134.2002

    Article  PubMed  CAS  Google Scholar 

  33. Smith PT, Landry ML, Carey H et al (1998) Papular-purpuric gloves and socks syndrome associated with acute parvovirus B19 infection: case report and review. Clin Infect Dis 27:164–168. doi:10.1086/514629

    Article  PubMed  CAS  Google Scholar 

  34. Soderlund-Venermo M, Hokynar K, Nieminen J et al (2002) Persistence of human parvovirus B19 in human tissues. Pathol Biol 50:307–316. doi:10.1016/S0369-8114(02)00307-3

    Article  PubMed  CAS  Google Scholar 

  35. Tomasini D, Tomasini CF, Cerri A et al (2004) Pityriasis lichenoides: a cytotoxic T-cell-mediated skin disorder. Evidence of human parvovirus B19 DNA in nine cases. J Cutan Pathol 31:531–538. doi:10.1111/j.0303-6987.2004.00186.x

    Article  PubMed  Google Scholar 

  36. Vonderheid EC, Bernengo MG (2003) The Sézary syndrome: hematologic criteria. Hematol Oncol Clin North Am 17:1367–1389. doi:10.1016/S0889-8588(03)00120-5

    Article  PubMed  Google Scholar 

  37. Vuorinen T, Lammintausta K, Kotilainen P et al (2002) Presence of parvovirus B19 DNA in chronic urticaric and healthy human skin. J Clin Virol 25:217–221. doi:10.1016/S1386-6532(02)00012-4

    Article  PubMed  CAS  Google Scholar 

  38. Willemze R, Jaffe ES, Burg G et al (2005) WHO-EORTC classification for cutaneous lymphomas. Blood 105:3768–3785. doi:10.1182/blood-2004-09-3502

    Article  PubMed  CAS  Google Scholar 

  39. Yazici AC, Aslan G, Baz K et al (2006) A high prevalence of parvovirus B19 DNA in patients with psoriasis. Arch Dermatol Res 298:231–235. doi:10.1007/s00403-006-0678-9

    Article  PubMed  CAS  Google Scholar 

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This study was supported in part by a grant from the Regione Piemonte-Ricerca Sanitaria Finalizzata, 2005 A288.

Conflict of interest statement

The authors state no conflict of interest.

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Authors and Affiliations

  1. Dermatology Section, Department of Biomedical Science and Human Oncology, Turin University, Via Cherasco 23, 10126, Turin, Italy

    Maria Teresa Fierro, Renata Ponti, Alessandra Comessatti, Mauro Novelli & Maria Grazia Bernengo

  2. Virology Unit, Department of Public Health and Microbiology, Turin University, Turin, Italy

    Francesca Sidoti, Cristina Costa, Massimiliano Bergallo, Chiara Merlino & Rossana Cavallo

  3. Department of Plastic Surgery and Burn Unit Skin Bank, CTO Hospital, Turin, Italy

    Mara Fumagalli

Authors
  1. Francesca Sidoti
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  2. Maria Teresa Fierro
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  8. Mauro Novelli
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  11. Maria Grazia Bernengo
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Corresponding author

Correspondence to Maria Teresa Fierro.

Additional information

F. Sidoti and M. T. Fierro contributed equally to this work.

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Sidoti, F., Fierro, M.T., Costa, C. et al. Prevalence and significance of human parvovirus variants in skin from primary cutaneous T cell lymphomas, inflammatory dermatoses and healthy subjects. Arch Dermatol Res 301, 647–652 (2009). https://doi.org/10.1007/s00403-009-0954-6

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  • DOI: https://doi.org/10.1007/s00403-009-0954-6

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