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Chitosan/gelatin hybrid nanogel containing doxorubicin as enzyme-responsive drug delivery system for breast cancer treatment

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Abstract

Here, chitosan/gelatin hybrid nanogel containing gold nanoparticles (CS/AuNPs@Gel) was prepared as an efficient enzyme-responsive carrier for doxorubicin (DOX) delivery. Nanogel was fabricated via ionic crosslinking of CS/AuNPs in the presence of gelatin as an enzyme-responsive moiety. The physicochemical properties of the final compounds were investigated by FT-IR, DLS, TEM, and UV–Vis spectroscopy. The mean size of the prepared CS/AuNPs and CS/AuNPs@Gel was around 83 nm and 119.3 nm with a zeta potential of 83.9 mV and 31.9 mV, respectively. The loading efficiency of DOX in the CS/AuNPs@Gel nanogel was about 56% and DOX was released from the nanogel in an enzyme-responsive manner. The cytotoxic assay on the MCF-7 cells revealed the nontoxicity of free nanogel, the effectiveness of drug-loaded nanogel (DN), and DN in the presence of an enzyme compared with the free DOX.

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Acknowledgements

Also, the authors are thankful to the Research Center for Pharmaceutical Nanotechnology at Tabriz University of Medical Sciences and Faculty of Chemical and Petroleum Engineering, University of Tabriz, for the technical supports.

Funding

The study was financially supported by the Research Vice-Chancellor of Tabriz University of Medical Sciences (Grant # 66349).

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Authors and Affiliations

  1. Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran

    Shaghayegh Aslzad, Elaheh Dalir Abdolahinia, Nazanin Amiryaghoubi & Marziyeh Fathi

  2. Faculty of Chemical and Petroleum Engineering, University of Tabriz, Tabriz, 51666-16471, Iran

    Parisa Heydari & Hamid Erfan-Niya

  3. Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Azam Safary

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  1. Shaghayegh Aslzad
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Aslzad, S., Heydari, P., Abdolahinia, E.D. et al. Chitosan/gelatin hybrid nanogel containing doxorubicin as enzyme-responsive drug delivery system for breast cancer treatment. Colloid Polym Sci 301, 273–281 (2023). https://doi.org/10.1007/s00396-023-05066-5

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