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Effect of barley supplementation on the fecal microbiota, caecal biochemistry, and key biomarkers of obesity and inflammation in obese db/db mice

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Abstract

Purpose

Barley is a low-glycemic index grain that can help diabetic and obese patients. The effect of barley intake depends on the host and the associated gut microbiota. This study investigated the effect of barley intake on the fecal microbiota, caecal biochemistry, and key biomarkers of obesity and inflammation.

Methods

Obese db/db mice were fed diets with and without barley during 8 weeks; lean mice were used as lean controls. Fecal microbiota was evaluated using 16S marker gene sequencing in a MiSeq instrument; several markers of caecal biochemistry, obesity, and inflammation were also evaluated using standard techniques.

Results

Bacterial richness (i.e., Operational Taxonomic Units) and Shannon diversity indexes were similar in all obese mice (with and without barley) and higher compared to lean controls. Barley intake was associated with increased abundances of Prevotella, Lactobacillus, and the fiber-degraders S24-7 (Candidatus Homeothermaceae) compared to both lean and obese controls. The analysis of unweighted UniFrac distances showed a separate clustering of samples for each experimental group, suggesting that consumption of barley contributed to a phylogenetically unique microbiota distinct from both obese and lean controls. Caecal butyrate concentrations were similar in all obese mice, while succinic acid was lower in the barley group compared to obese controls. Barley intake was also associated with lower plasma insulin and resistin levels compared to obese controls.

Conclusions

This study shows that barley intake is associated with a different fecal microbiota, caecal biochemistry, and obesity biomarkers in db/db mice that tend to be more similar to lean controls.

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Acknowledgements

DAM acknowledges the Peter J. Shields Endowed Chair. The authors would like to express their deepest gratitude to the QIIME and PICRUSt Help Forums for all the support provided. The authors would also like to thank Alejandra Mencia for her technical assistance in the analysis of blood parameters.

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  1. Giuliana Noratto

    Present address: Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA

Authors and Affiliations

  1. Research Group Medical Eco-Biology, Faculty of Veterinary Medicine, Universidad Autónoma de Nuevo León, General Escobedo, Nuevo León, Mexico

    Jose F. Garcia-Mazcorro

  2. Department of Food Science and Technology, University of California, Davis, CA, USA

    David A. Mills

  3. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, USA

    Kevin Murphy

  4. School of Food Science, Washington State University, Pullman, WA, USA

    Giuliana Noratto

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  1. Jose F. Garcia-Mazcorro
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Correspondence to Giuliana Noratto.

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Garcia-Mazcorro, J.F., Mills, D.A., Murphy, K. et al. Effect of barley supplementation on the fecal microbiota, caecal biochemistry, and key biomarkers of obesity and inflammation in obese db/db mice. Eur J Nutr 57, 2513–2528 (2018). https://doi.org/10.1007/s00394-017-1523-y

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