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A combination of scGOS/lcFOS with Bifidobacterium breve M-16V protects suckling rats from rotavirus gastroenteritis

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Abstract

Purpose

Rotavirus (RV) is the leading cause of severe diarrhoea among infants and young children, and although more standardized studies are needed, there is evidence that probiotics can help to fight against RV and other infectious and intestinal pathologies. On the other hand, the effects of prebiotics have not been properly addressed in the context of an RV infection. The aim of this study was to demonstrate a protective role for a specific scGOS/lcFOS 9:1 prebiotic mixture (PRE) separately, the probiotic Bifidobacterium breve M-16V (PRO) separately and the combination of the prebiotic mixture and the probiotic (synbiotic, SYN) in a suckling rat RV infection model.

Methods

The animals received the intervention from the 3rd to the 21st day of life by oral gavage. On day 7, RV was orally administered. Clinical parameters and immune response were evaluated.

Results

The intervention with the PRO reduced the incidence, severity and duration of the diarrhoea (p < 0.05). The PRE and SYN products improved clinical parameters as well, but a change in stool consistency induced by the PRE intervention hindered the observation of this effect. Both the PRE and the SYN, but not the PRO, significantly reduced viral shedding. All interventions modulated the specific antibody response in serum and intestinal washes at day 14 and 21 of life.

Conclusions

A daily supplement of a scGOS/lcFOS 9:1 prebiotic mixture, Bifidobacterium breve M-16V or a combination of both is highly effective in modulating RV-induced diarrhoea in this preclinical model.

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Acknowledgments

This study was supported by a Nutricia Research grant in collaboration with the Fundació Bosch i Gimpera (FBG306349). MRA was the recipient of a fellowship from the Fundació Pedro i Pons. The authors thank Dr. Adele Costabile for her help and advice with the HPLC.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Pharmacy, University of Barcelona, Av. Joan XXIII s/n, 08028, Barcelona, Spain

    M. Rigo-Adrover, S. Saldaña-Ruíz, A. Franch, M. Castell & F. J. Pérez-Cano

  2. Nutrition and Food Safety Research Institute (INSA), Barcelona, Spain

    M. Rigo-Adrover, S. Saldaña-Ruíz, A. Franch, M. Castell & F. J. Pérez-Cano

  3. Nutricia Research, Utrecht, The Netherlands

    K. van Limpt, K. Knipping, J. Garssen & J. Knol

  4. Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, The Netherlands

    K. Knipping & J. Garssen

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  1. M. Rigo-Adrover
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  2. S. Saldaña-Ruíz
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  3. K. van Limpt
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  4. K. Knipping
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  6. J. Knol
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  7. A. Franch
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  8. M. Castell
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  9. F. J. Pérez-Cano
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Correspondence to F. J. Pérez-Cano.

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Conflict of interest

The authors declare that they have a financial relationship with the organization that sponsored the research. K. van Limpt, K. Knipping, J. Garssen and J. Knol are employees of Nutricia Research B.V. The other authors declare that they have no conflict of interest.

Ethical standards

The studies were conducted in accordance with the institutional guidelines for the care and use of laboratory animals established by the Ethics Committee for Animal Experimentation of the University of Barcelona and the Catalonian Government (CEEA-UB Ref.165/11, DAAM: 5871).

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Rigo-Adrover, M., Saldaña-Ruíz, S., van Limpt, K. et al. A combination of scGOS/lcFOS with Bifidobacterium breve M-16V protects suckling rats from rotavirus gastroenteritis. Eur J Nutr 56, 1657–1670 (2017). https://doi.org/10.1007/s00394-016-1213-1

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  • DOI: https://doi.org/10.1007/s00394-016-1213-1

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