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Maternal high-fat diet is associated with altered pancreatic remodelling in mice offspring

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Abstract

Purpose

To investigate whether a maternal high-fat diet (HF) during pregnancy and/or suckling periods predisposes adult C57BL/6 mice offspring to morphological pancreatic modifications.

Methods

Male pups were divided into 5 groups: SC (standard chow)—from dams fed SC during gestation and lactation, maintaining an SC diet from postweaning to adulthood; G—from dams fed HF diets during gestation; L—from dams fed HF diets during lactation; GL—from dams fed HF diets during gestation and lactation; and GL/HF—from dams fed HF diets during gestation and lactation, maintaining an HF diet from postweaning to adulthood. We analysed body mass (BM), plasma insulin, pancreas and adipose tissue structures.

Results

During the entire experiment, the SC group had the lowest BM. However, GL/HF offspring were heavier than the other groups. This weight gain was also accompanied by adipocyte hypertrophy. At 3 months, G offspring showed an increased insulin levels and impairment in carbohydrates metabolism. Furthermore, pancreatic islets were hypertrophied in G, GL and GL/HF offspring in comparison with SC offspring.

Conclusion

HF diet administration during the gestation period is more harmful than during the lactation period, exerting deleterious effects on pancreatic morphology in addition to larger fat deposits in adult mice offspring.

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Acknowledgments

This research was supported by grants from the Brazilian agencies CNPq (Conselho Nacional de Ciencia e Tecnologia) and FAPERJ (Fundaçao para o Amparo a Pesquisa do Estado do Rio de Janeiro). The authors would like to thank Thatiany Marinho and Angelica Figueiredo for their technical assistance.

Conflicts of interest

None.

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Authors and Affiliations

  1. Laboratório de Morfometria, Metabolismo e Doença Cardiovascular, Centro Biomédico, Universidade do Estado do Rio de Janeiro, Av. 28 de Setembro 87 (fds), 20551-030, Rio de Janeiro, RJ, Brazil

    Bianca Martins Gregorio, Vanessa Souza-Mello, Carlos Alberto Mandarim-de-Lacerda & Marcia Barbosa Aguila

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  1. Bianca Martins Gregorio
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  2. Vanessa Souza-Mello
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Correspondence to Marcia Barbosa Aguila.

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Gregorio, B.M., Souza-Mello, V., Mandarim-de-Lacerda, C.A. et al. Maternal high-fat diet is associated with altered pancreatic remodelling in mice offspring. Eur J Nutr 52, 759–769 (2013). https://doi.org/10.1007/s00394-012-0382-9

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  • DOI: https://doi.org/10.1007/s00394-012-0382-9

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