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Dehydroepiandrosterone (DHEA) prevents the prostanoid imbalance in mesenteric bed of fructose-induced hypertensive rats

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Abstract

Background

In previous studies we reported an altered prostanoid (PR) release-pattern in mesenteric vessels in fructose (F)-overloaded rats, an experimental model of insulin resistance and hypertension. Dehydroepiandrosterone (DHEA) and its precursor Dehydroepiandrosterone sulfate (DHEA-S) are the most abundant circulating steroid hormones produced by the adrenal and recent studies in both cells and animals suggest that DHEA may have acute non-genomic actions that mimic both metabolic and vascular actions of insulin.

Aim of the study

This study was to analyze in F-overloaded rats, the effects of DHEA treatment on arterial blood pressure and the PR production in mesenteric vessels and aorta.

Methods

Male 6 week-old Sprague–Dawley rats were randomly divided in four groups: a control group (C), a DHEA (30 mg/kg/sc/48 h)-treated group (D), a fructose (10% w/v in drinking water)-fed group (F), and both treatments simultaneously group (FD). The systolic blood pressure (SBP) was measured by tail cuff method and glycemia and triglyderidemia were measured by enzymatic assays. The mesenteric beds of all groups were dissected, and incubated in Krebs solution. The PR released were measured by HPLC.

Results

F overload increased SBP and triglyceridemia and decreased the mesenteric vasodilatory PR release. DHEA treatment prevented the increment in SBP and triglyceridemia and decreased vasoconstrictor PR in F-treated rats.

Conclusion

DHEA normalize the PGI2/TX ratio, diminished in F-overloaded rats, through the decrease in thromboxane (TX) production and this could be one of the mechanisms by which DHEA prevented the slight hypertension in F-animals.

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Acknowledgments

This study was suppored by grants from the Secretaría de Ciencia y Técnica of the Universidad de Buenos Aires (Code B 109) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), PIP 2109/00 and PIP 5497. We gratefully acknowledge the valuable assistance of Dr. Belisario Fernandez in discussing and revising the manuscript.

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Authors and Affiliations

  1. Dept. of Pharmatechnology I, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Buenos Aires, Argentina

    Horacio A. Peredo

  2. CONICET, Buenos Aires, Argentina

    Horacio A. Peredo, Marcos Mayer & Andrea Carranza

  3. Dept. of Physiopathology, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Buenos Aires, Argentina

    Marcos Mayer & Andrea Carranza

  4. Dept. of Macro and Microscopy Anatomy, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Buenos Aires, Argentina

    Ileana R. Faya & Ana M. Puyó

  5. Facultad de Medicina, Instituto de Neurociencias, Universidad de Buenos Aires, Paraguay 2153, Piso 1, Sector Uriburu (C1121ABG), Buenos Aires, Argentina

    Andrea Carranza

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  1. Horacio A. Peredo
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  2. Marcos Mayer
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Correspondence to Andrea Carranza.

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Peredo, H.A., Mayer, M., Faya, I.R. et al. Dehydroepiandrosterone (DHEA) prevents the prostanoid imbalance in mesenteric bed of fructose-induced hypertensive rats. Eur J Nutr 47, 349–356 (2008). https://doi.org/10.1007/s00394-008-0734-7

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