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Assessment of microsatellite instability in colorectal carcinoma at an Indian center

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Aim

The purpose of the present study was to evaluate the frequency of microsatellite instability (MSI) in colorectal cancers in an Indian cohort.

Materials and methods

Paraffin embedded tissue samples of colorectal cancers from 46 patients were assessed for mismatch repair protein expression (hMLH1 and hMSH2) by immunohistochemistry. Subsequently, MSI analysis was done after PCR amplification of five Bethesda markers.

Results

Amongst 46 cases studied, only 5 patients (10.8%) showed MSI. Out of these, two (4.3%) had high microsatellite instability (MSI-H) and three (6.5%) showed low microsatellite instability (MSI-L). Out of 46 cases, 41 were microsatellite stable (MSS). In the 46 cases tested by immunohistochemistry, 7 (15.7%) showed the absence of hMLH1 and 1 case showed the absence of hMSH2.

Conclusion

Our study indicates a similar rate of incidence of MSI in colorectal cancers in the Indian cohort compared to the West (10–15%) despite lower incidence of colorectal cancers and predominance of rectosigmoid tumors in the Indian population.

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Abbreviations

MSI:

microsatellite instability

CRC:

colorectal cancer

gDNA:

genomic DNA

MSS:

microsatellite stable

MSI-L:

microsatellite instability low

MSI-H:

microsatellite instability high

HNPCC:

hereditary non-polyposis colorectal cancer

H & E:

hematoxylin and eosin

IHC:

immunohistochemistry

CAP:

College of American Pathologists

hMLH1:

human mutL homolog 1

hMSH2:

human mutS homolog 2

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Acknowledgement

We thank Aruna Korlimarla, Shivangi Wani, Joseph Manoj Victor, and Sriranga Raju for their valuable technical assistance.

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Authors and Affiliations

  1. Triesta Sciences, IPHCR Building, St. John’s Medical College Campus, Opposite Koramangala BDA Complex, Bangalore, 560034, India

    Vijay Pandey, Jyothi S. Prabhu, Payal K., Veena Rajan & Sridhar T. S.

  2. Asian Institute of Oncology, S.L. Raheja Hospital, Raheja Hospital Road, Mahim, Mumbai, 400016, India

    C. Deepak, Shubhada Barde, P. Jagannath & Anita Borges

Authors
  1. Vijay Pandey
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  2. Jyothi S. Prabhu
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  3. Payal K.
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  4. Veena Rajan
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  5. C. Deepak
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  6. Shubhada Barde
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  7. P. Jagannath
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  8. Anita Borges
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  9. Sridhar T. S.
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Corresponding author

Correspondence to Vijay Pandey.

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Pandey, V., Prabhu, J.S., K., P. et al. Assessment of microsatellite instability in colorectal carcinoma at an Indian center. Int J Colorectal Dis 22, 777–782 (2007). https://doi.org/10.1007/s00384-006-0241-3

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  • DOI: https://doi.org/10.1007/s00384-006-0241-3

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