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Ketotifen ameliorates development of fibrosis in alkali burns of the esophagus

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Abstract

An experimental study was performed to investigate the efficacy of ketotifen, which is a mast cell stabilizer and histamine H1-receptor antagonist, on the prevention of stricture development after esophageal caustic injuries in the rat. Caustic esophageal burn was created by applying 37.5% NaOH to the distal esophagus. Forty rats were divided into four equal groups. Group A (sham) animals were uninjured. Group B rats were injured but untreated. Group C rats were injured and received ketotifen (1 mg/kg/day) via the oral route. Group D rats were injured and received ketotifen (1 mg/kg/day) via the intraperitoneal route. Efficacy of the treatment was assessed on day 28 by measuring the stenosis index and histopathologic damage score and biochemically by determining tissue hydroxyproline content. The stenosis index in group B (0.93±0.22) was significantly increased compared with group A (0.39±0.06, p <0.05), group C (0.42±0.09, p <0.05), and group D (0.35±0.07, p <0.05). The hydroxyproline level (µg/mg wet tissue) was significantly increased in group B (1.31±0.08, p <0.05) compared with group A (0.69±0.16, p <0.05), group C (1.06±0.16, p <0.05), and group D (0.95±0.12, p <0.05). In group B the histopathologic damage score was significantly higher than in groups C (p <0.05) and D (p <0.05). There was no significant difference between group C and group D in terms of all parameters evaluated. Treatment with ketotifen decreased tissue hydroxyproline levels, histological damage, and the stenosis index. We conclude that ketotifen has a preventive effect in the development of fibrosis in an experimental model of corrosive esophagitis in rats.

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Acknowledgements

The authors would like to thank Dr. Timur Kose, Ege University Faculty of Engineering Division of Computer Sciences, for statistical analysis. We also thank Mr. Ismail Zonguldak, technician, Ege University School of Medicine, Department of Experimental Surgery, for technical assistance during the experimental procedures.

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Authors and Affiliations

  1. Department of Gastroenterology, Adnan Menderes University School of Medicine, 09100, Aydin, Turkey

    Vahit Yukselen & Ali Onder Karaoglu

  2. Faculty of Medicine Department of Gastroenterology, Ege University, Izmir, Turkey

    Omer Ozutemiz

  3. Department of Biochemistry, Adnan Menderes University School of Medicine, Aydin, Turkey

    Cigdem Yenisey

  4. Faculty of Medicine Department of Pathology, Ege University, Izmir, Turkey

    Muge Tuncyurek

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  1. Vahit Yukselen
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  2. Ali Onder Karaoglu
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  5. Muge Tuncyurek
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Correspondence to Vahit Yukselen.

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Yukselen, V., Karaoglu, A.O., Ozutemiz, O. et al. Ketotifen ameliorates development of fibrosis in alkali burns of the esophagus. Ped Surgery Int 20, 429–433 (2004). https://doi.org/10.1007/s00383-004-1170-2

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