Abstract
In adult amphibian skin, Na+ crosses from outside to inside. This Na+ transport can be measured as the amiloride-blockable short-circuit current (SCC) across the skin. We investigated the effects of arginine vasotocin (AVT) and mesotocin (MT), and those of antagonists of the vasopressin and oxytocin receptors, on the SCC across Hyla japonica skin. (1) Both AVT (100 pmol/L or more) and MT (1 nmol/L or more) increased the SCC. (2) The AVT- and MT-induced increases in SCC recovered with time (downregulation). (3) These AVT/MT-induced effects were blocked by application of OPC-31260 (vasopressin V2-receptor antagonist). (4) The OPC-31260 concentration needed to block the AVT-induced response was lower upon post-application (after application of agonist) than upon pre-application (before application of agonist), suggesting the number of receptors may have decreased after AVT application. (5) Upon repeated application of AVT (100 pmol/L), the induced SCC increase did not differ significantly between the 1st and 2nd applications. (6) The time to reach the half-maximum value of the AVT-induced or MT-induced increase in SCC was not significantly different between washout and post-application of OPC-31260, suggesting that post-application of OPC-31260 cleared AVT and MT from their receptors. The effects of AVT, MT, and their antagonists in H. japonica, which is adapted to a terrestrial habitat, are compared with our previously published data on Rana catesbeiana (=Lithobates catesbeianus), which is adapted to a semiaquatic habitat.
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Acknowledgments
All procedures complied with the relevant Japanese law governing animal experimentation (Law No. 105, Notification No. 000398). We are greatly indebted to Dr. Toyoki Mori and his co-workers at Otsuka Pharmaceutical Co. Ltd. for their gifts of OPC-21268 and OPC-31260. This research was supported in part by a Saitama Medical University Internal Grant (No. 21-1-2-11).
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Communicated by G. Heldmaier.
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Takada, M., Fujimaki-Aoba, K. & Hokari, S. Vasotocin- and mesotocin-induced increases in short-circuit current across tree frog skin. J Comp Physiol B 181, 239–248 (2011). https://doi.org/10.1007/s00360-010-0523-5
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DOI: https://doi.org/10.1007/s00360-010-0523-5