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Molecular cloning and characterization of SoxB2 gene from Zhikong scallop Chlamys farreri

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Abstract

The Sox proteins play critical roles during the development of animals, including sex determination and central nervous system development. In this study, the SoxB2 gene was cloned from a mollusk, the Zhikong scallop (Chlamys farreri), and characterized with respect to phylogeny and tissue distribution. The full-length cDNA and genomic DNA sequences of C. farreri SoxB2 (Cf SoxB2) were obtained by rapid amplification of cDNA ends and genome walking, respectively, using a partial cDNA fragment from the highly conserved DNA-binding domain, i.e., the High Mobility Group (HMG) box. The full-length cDNA sequence of Cf SoxB2 was 2 048 bp and encoded 268 amino acids protein. The genomic sequence was 5 551 bp in length with only one exon. Several conserved elements, such as the TATA-box, GC-box, CAAT-box, GATA-box, and Sox/sry-sex/testis-determining and related HMG box factors, were found in the promoter region. Furthermore, real-time quantitative reverse transcription PCR assays were carried out to assess the mRNA expression of Cf SoxB 2 in different tissues. SoxB2 was highly expressed in the mantle, moderately in the digestive gland and gill, and weakly expressed in the gonad, kidney and adductor muscle. In male and female gonads at different developmental stages of reproduction, the expression levels of Cf SoxB2 were similar. Considering the specific expression and roles of SoxB 2 in other animals, in particular vertebrates, and the fact that there are many pallial nerves in the mantle, cerebral ganglia in the digestive gland and gill nerves in gill, we propose a possible essential role in nervous tissue function for Sox B 2 in C. farreri.

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Authors and Affiliations

  1. Key Laboratory of Marine Genetics and Breeding (MGB), Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Yan He  (贺艳), Zhenmin Bao  (包振民), Huihui Guo  (郭慧慧), Yueyue Zhang  (张月月), Lingling Zhang  (张玲玲), Shi Wang  (王师), Jingjie Hu  (胡景杰) & Xiaoli Hu  (胡晓丽)

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  1. Yan He  (贺艳)
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  2. Zhenmin Bao  (包振民)
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  3. Huihui Guo  (郭慧慧)
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  4. Yueyue Zhang  (张月月)
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  5. Lingling Zhang  (张玲玲)
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  6. Shi Wang  (王师)
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  7. Jingjie Hu  (胡景杰)
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  8. Xiaoli Hu  (胡晓丽)
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Correspondence to Xiaoli Hu  (胡晓丽).

Additional information

Supported by the National Natural Science Foundation of China (Nos. 31072190, 30972239), the National Basic Research Program of China (973 Program) (No. 2010CB126406), the Taishan Scholar Program of Shandong Province, the Doctoral Fund of Ministry of Education of China (No. 20100132110014), and the Natural Science Foundation of Shandong Province (No. ZR2009DM019)

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He, Y., Bao, Z., Guo, H. et al. Molecular cloning and characterization of SoxB2 gene from Zhikong scallop Chlamys farreri . Chin. J. Ocean. Limnol. 31, 1216–1225 (2013). https://doi.org/10.1007/s00343-013-3039-5

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  • DOI: https://doi.org/10.1007/s00343-013-3039-5

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