Abstract
A nonlinear dynamic phase-contrast stereoscope has been developed for the measurement of all three velocity components of a microfluidic flow field. The stereoscope system captures simultaneously two images of different off-axis views of the same region of interest in a microflow, seeded with tracer particles. Two independent photorefractive two-beam coupling novelty filters, one in each stereoscope channel, are employed to enhance the contrast of tracer particle images. A subsequently applied particle tracking algorithm extracts the velocity information from the images, and in first experiments the axial velocity components could be determined with an error of less than 5%. Finally we report on the determination of the velocity field in a rectangular microchannel with a 170 μm high microstep with the dynamic phase-contrast stereoscope.
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References
A. Manz, N. Graber, H.M. Widmer, Sens. Actuators B 1, 244 (1990)
O. Geschke, H. Klank, P. Tellman (eds.), Microsystem Engineering of Lab-on-a-Chip Devices (Wiley, New York, 2004)
G.M. Whitesides, Nature 442, 368 (2006)
J. Santiago, S. Wereley, C. Meinhart, D. Beebe, R. Adrian, Exp. Fluids 25, 316 (1998)
C.D. Meinhart, S.T. Wereley, J.G. Santiago, Exp. Fluids 27, 414 (1999)
D. Sinton, Microfluid. Nanofluid. 1, 2 (2004)
S. Jian, E. Malkiel, J. Katz, Appl. Opt. 42, 235 (2003)
C. Yang, H. Chuang, Exp. Fluids 39, 385–396 (2005)
B. Ovryn, Exp. Fluids 29, S175–184 (2000)
J. Park, K. Kihm, Exp. Fluids 40, 491–499 (2006)
S.D. Peterson, H.S. Chuang, S.T. Wereley, Meas. Sci. Technol. 19, 115406 (2008)
M.R. Bown, J.M. MacInnes, R.W.K. Allen, W.B.J. Zimmerman, Meas. Sci. Technol. 17, 2175 (2006)
R. Lindken, J. Westerweel, B. Wieneke, Exp. Fluids 41, 161 (2006)
M.R. Bown, J.M. MacInnes, R.W.K. Allen, Exp. Fluids 42, 197 (2007)
W.J. Parak, T. Pellegrino, C. Plank, Nanotechnology 16, R9 (2005)
H. Petermeier, W. Kowalczyk, A. Delgado, C. Denz, F. Holtmann, Exp. Fluids 42, 611 (2007)
Y. Sugii, S. Nishio, K. Okamoto, Physiol. Meas. 23, 403 (2002)
D. Anderson, J. Feinberg, IEEE J. Quantum Electron. 25, 635 (1989)
M. Sedlatschek, J. Trumpfheller, J. Hartmann, M. Müller, C. Denz, T. Tschudi, Appl. Phys. B 68, 1047 (1999)
V.V. Krishnamachari, C. Denz, J. Opt. A: Pure Appl. Opt. 5, 239 (2003)
V.V. Krishnamachari, C. Denz, Appl. Phys. B 79, 497 (2004)
R.S. Cudney, R.M. Pierce, J. Feinberg, Nature 332, 424 (1988)
M. Sedlatschek, T. Rauch, C. Denz, T. Tschudi, Opt. Mater. 4, 376 (1995)
M. Woerdemann, F. Holtmann, C. Denz, J. Opt. A: Pure Appl. Opt. 11, 034010 (2009)
M. Woerdemann, F. Holtmann, C. Denz, Appl. Phys. Lett. 93, 021108 (2008)
F. Holtmann, M. Woerdemann, C. Denz, Nonlinear dynamic phase contrast microscopy for microflow analysis, in Imaging Measurement Methods for Flow Analysis (Springer, Berlin, 2009)
V.V. Krishnamachari, O. Grothe, H. Deitmar, C. Denz, Appl. Phys. Lett. 87, 071105 (2005)
F. Holtmann, M. Eversloh, C. Denz, J. Opt. A: Pure Appl. Opt. 11, 034014 (2009)
P. Yeh, Introduction to Photorefractive Nonlinear Optics (Wiley, New York, 1993)
Y.G. Guezennec, R.S. Brodkey, N. Trigui, J.C. Kent, Exp. Fluids 17, 209 (1994)
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Holtmann, F., Oevermann, M. & Denz, C. Dynamic phase-contrast stereoscopy for microflow velocimetry. Appl. Phys. B 95, 633–636 (2009). https://doi.org/10.1007/s00340-009-3500-5
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DOI: https://doi.org/10.1007/s00340-009-3500-5