Abstract
A number of American mink phenotypes display a range of brownish colours. One of these phenotypes, namely American Palomino (b P b P ) (AP) has been found to be associated with the tyrosinase-related protein 1 (TYRP1) gene by genotyping microsatellite markers in one sire family. Trials for amplifying the genomic DNA and cDNA at the beginning of intron 2 of AP TYRP1 revealed the presence of a large insertion of approximately eight kb. The insertion most likely disrupts different elements necessary for the splicing of intron 2 of the TYRP1 gene. In AP RNAseq data indicate, however, the presence of the wild-type (wt) transcript at very low levels and Western blot reveals three products when using an antibody raised against middle part of the TYRP1 protein. One individual from another brown mink phenotype—commercially named Dawn—was also investigated at the molecular level by long-range PCR and the same size insertion appears to be present. By this we suggest that certain modifiers of TYRP1 would induce different brown colour degradation, which results in at least two different phases of brown.
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Acknowledgments
This work was supported from private funding. We are thankful to Tina B. Neergaard Mahler and Minna Jakobsen for technical assistance. We acknowledge Boye Pedersen who is in charge of the KU mink farm. We also acknowledge Jesper Clausen for providing some of the pictures of the mink.
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The authors declare not having any conflict of interest. The authors are investigation scientists and although they work with a politically controversial fur animal species, herein they are only supporters of the science. Their political view might therefore differ from the line of research they are engaged in.
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Brown spectrum in American mink. American Palomino, Dawn and wild type are indicated. Photograph: Jesper Clausen (TIFF 2174 kb)
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Mink phenotypes homozygous for the TYRP1 mutation: (A) American Palomino b P b P and (B) Dawn besides a wild type mink (C) (TIFF 883 kb)
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Pedigrees segregating informatively for American Palomino phenotype in American mink. Circles represent females, squares represent males, rombes unrecorded sex. Solid symbols represent American Palomino homozygotes (bb) and clear symbols represent wild type (homo- or heterozygous; BB or Bb) individuals (TIFF 683 kb)
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Cirera, S., Markakis, M.N., Kristiansen, T. et al. A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink. Mamm Genome 27, 135–143 (2016). https://doi.org/10.1007/s00335-016-9620-4
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DOI: https://doi.org/10.1007/s00335-016-9620-4